Home LiteratureArticle Details
PMID: 4000950 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of beta A chromatin.

Nucleic acids research ·Vol. 13 ·No. 6 ·1985-03-25 ·Pages 1977-95

Ferenz CR, Nelson DA

Abstract

The solubility of adult beta-globin chromatin (beta A chromatin) from immature chicken red blood cells can be controlled by the presence or absence of n-butyrate in a cell incubation medium. In the absence of n-butyrate, only a small percentage (approximately 4%) of the total beta A chromatin is in a soluble chromatin fraction following micrococcal nuclease digestion and centrifugation. This percentage increases to approximately 40-45% of the beta A chromatin if cells are incubated 1 hour in the presence of 10 mM sodium n-butyrate. The highest yield and enrichment of solubilized beta A chromatin is attained when 1-4% of the DNA is rendered acid soluble, and in buffers containing 1.5 - 5 mM MgCl2. The soluble beta A nucleohistone is nucleosome oligomer size (contains DNA 250-600 bases in length) and can be separated from soluble, transcriptionally inert mononucleosomes by agarose A-5m exclusion chromatography. The enhanced solubility appears to be specific for transcriptionally active chromatin. Whereas 40-45% of the beta A chromatin is recovered in the supernatant fraction from n-butyrate incubated immature erythrocytes, nucleohistone containing ovalbumin DNA sequences remains insoluble.

MeSH Terms
Acetylation Animals Butyrates/pharmacology Butyric Acid Chickens Chromatin/isolation & purification,physiology Gene Expression Regulation/drug effects Globins/genetics Histones/physiology Magnesium/pharmacology Micrococcal Nuclease Solubility Transcription, Genetic
Chemicals
Butyrates Chromatin Histones Butyric Acid Globins Micrococcal Nuclease Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferenz C R
Nelson D A
References (54)
54 references, click to expand
  1. Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone.
    J Biol Chem. 1968 Oct 10;243(19):5018-22 PMID: 5679978
  2. Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.
    J Biol Chem. 1968 Dec 25;243(24):6361-6 PMID: 5726891
  3. Calf and pea histone IV. 3. Complete amino acid sequence of pea seedling histone IV; comparison with the homologous calf thymus histone.
    J Biol Chem. 1969 Oct 25;244(20):5669-79 PMID: 5388597
  4. A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes.
    Biochemistry. 1973 Feb 27;12(5):783-91 PMID: 4568767
  5. Enzymatic modifications and their possible roles in regulating the binding of basic proteins to DNA and in controlling chromosomal structure.
    Cold Spring Harb Symp Quant Biol. 1974;38:803-19 PMID: 4524789
  6. Partial purification of the template-active fraction of chromatin: a preliminary report.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2193-7 PMID: 4526205
  7. Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells.
    Cell. 1975 Jul;5(3):319-22 PMID: 1056809
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  10. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  11. n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells.
    Nature. 1977 Aug 4;268(5619):462-4 PMID: 268489
  12. Suppression of histone deacetylation in vivo and in vitro by sodium butyrate.
    J Biol Chem. 1978 May 25;253(10):3364-6 PMID: 649576
  13. Structure of chromatin containing extensively acetylated H3 and H4.
    Cell. 1978 Apr;13(4):691-9 PMID: 657272
  14. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  15. Sodium butyrate inhibits histone deacetylation in cultured cells.
    Cell. 1978 May;14(1):105-13 PMID: 667927
  16. The effect of sodium butyrate on histone modification.
    Cell. 1978 May;14(1):115-21 PMID: 667928
  17. Turnover of histone acetyl groups in cultured cells is inhibited by sodium butyrate.
    FEBS Lett. 1978 Jul 1;91(1):117-20 PMID: 668901
  18. Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43 PMID: 276864
  19. Histones and chromatin structure in Tetrahymena macro- and micronuclei.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:493-503 PMID: 98284
  20. Acetylated histone H4 is preferentially associated with template-active chromatin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3574-7 PMID: 278972
  21. DNAse I preferentially digests chromatin containing hyperacetylated histones.
    Biochem Biophys Res Commun. 1978 Jun 29;82(4):1346-53 PMID: 697799
  22. Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion.
    Cell. 1978 Sep;15(1):141-50 PMID: 699037
  23. Fractionation of nucleosomes by salt elution from micrococcal nuclease-digested nuclei.
    J Cell Biol. 1978 Oct;79(1):97-109 PMID: 701381
  24. Partial purification of transcriptionally active nucleosomes from trout testis cells.
    Nucleic Acids Res. 1978 Nov;5(11):4155-63 PMID: 569293
  25. Transfer of small DNA fragments from polyacrylamide gels to diazobenzyloxymethyl-paper and detection by hybridization with DNA probes.
    Biochem Biophys Res Commun. 1978 Dec 14;85(3):1104-12 PMID: 216362
  26. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 PMID: 284387
  27. Histone deacetylation in nuclei isolated from hepatoma tissue culture cells. Inhibition by sodium butyrate.
    Biochim Biophys Acta. 1979 Feb 27;561(2):517-25 PMID: 427171
  28. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  29. Interaction of HMG 14 and 17 with actively transcribed genes.
    Cell. 1980 Jan;19(1):289-301 PMID: 6244103
  30. Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I.
    Cell. 1980 Jun;20(2):451-60 PMID: 7388947
  31. The identification of distinct populations of acetylated histone.
    J Biol Chem. 1980 Oct 10;255(19):9110-6 PMID: 7410415
  32. An endonuclease activity of chicken erythrocyte nuclei and mononucleosomes.
    J Biol Chem. 1980 Nov 10;255(21):10542-5 PMID: 6253479
  33. Segregation of rapidly acetylated histones into a chromatin fraction released from intact nuclei by the action of micrococcal nuclease.
    Nucleic Acids Res. 1980 Apr 25;8(8):1745-63 PMID: 7433128
  34. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  35. The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.
    J Biol Chem. 1981 Apr 10;256(7):3313-8 PMID: 6259161
  36. Histone modifications in the yeast S. Cerevisiae.
    Nucleic Acids Res. 1981 Jul 10;9(13):3205-16 PMID: 7024912
  37. Manifold effects of sodium butyrate on nuclear function. Selective and reversible inhibition of phosphorylation of histones H1 and H2A and impaired methylation of lysine and arginine residues in nuclear protein fractions.
    J Biol Chem. 1981 Sep 25;256(18):9612-21 PMID: 6270094
  38. Chemical composition of nucleosomes among domains of calf thymus chromatin differing in micrococcal nuclease accessibility and solubility properties.
    J Biol Chem. 1981 Dec 10;256(23):12574-80 PMID: 6457837
  39. Modulation of nucleosome structure by histone subtypes in sea urchin embryos.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6803-7 PMID: 6947254
  40. Release of a globin gene enriched chromatin fraction from chicken erythrocyte nuclei following DNase II digestion.
    Nucleic Acids Res. 1981 Dec 11;9(23):6471-85 PMID: 6275364
  41. Histone acetylation in baker's yeast. Maintenance of the hyperacetylated configuration in log phase protoplasts.
    J Biol Chem. 1982 Feb 25;257(4):1565-8 PMID: 7035452
  42. Accessibility of newly synthesized chromatin to histone acetylase.
    J Biol Chem. 1982 Apr 10;257(7):3945-9 PMID: 7061519
  43. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells.
    Cell. 1982 Jan;28(1):99-106 PMID: 7066988
  44. Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation.
    J Biol Chem. 1982 Jul 10;257(13):7336-47 PMID: 7085629
  45. Distribution of acetylated forms of nucleosomal histones in fractionated chromatin.
    Arch Biochem Biophys. 1982 Jun;216(1):88-92 PMID: 7103517
  46. Endogenous nuclease. Properties and effects on transcribed genes in chromatin.
    J Biol Chem. 1982 Nov 10;257(21):13009-17 PMID: 7130192
  47. The nuclease sensitivity of active genes.
    Nucleic Acids Res. 1983 Feb 11;11(3):753-72 PMID: 6300766
  48. Modulations of prolactin and growth hormone gene expression and chromatin structure in cultured rat pituitary cells.
    Nucleic Acids Res. 1983 Feb 11;11(3):823-35 PMID: 6687634
  49. The association of transcriptionally active genes with the nuclear matrix of the chicken oviduct.
    Nucleic Acids Res. 1983 Aug 11;11(15):5113-30 PMID: 6192395
  50. Histone deacetylation is required for the maturation of newly replicated chromatin.
    J Biol Chem. 1983 Oct 25;258(20):12675-84 PMID: 6226660
  51. Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):304-7 PMID: 6582488
  52. Enrichment of transcribed and newly replicated DNA in soluble chromatin released from nuclei by mild micrococcal nuclease digestion.
    Biochim Biophys Acta. 1984 Jun 16;782(2):202-9 PMID: 6547059
  53. Differential salt fractionation of active and inactive genomic domains in chicken erythrocyte.
    J Biol Chem. 1984 Jul 10;259(13):8558-63 PMID: 6736042
  54. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.6.1977
Published
1985-03-25
Pages
1977-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341129
Subset
IM
Grants
NIGMS NIH HHS · GM29442 · United States
External Links
PubMed source DOI: 10.1093/nar/13.6.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2