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PMID: 4024871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The distribution of laminin in human brain tumors: an immunohistochemical study.

Acta neuropathologica ·Vol. 67 ·No. 1-2 ·1985-00-00 ·Pages 51-7

Giordana MT, Germano I, Giaccone G, Mauro A, Migheli A, Schiffer D

Abstract

The immunohistochemical distribution of laminin in 70 cases of primary and secondary brain tumors has been studied. The sections were fixed in Carnoy, embedded in paraffin, and treated with collagenase before applying the specific immune serum. In normal neural tissue and in gliomas, the distribution of laminin overlaps with that of basement membrane, as is known from observations on ultrastructure in the literature. The overlapping was observed also in neurinomas, papillomas of the choroid plexus, ependymomas, and meningiomas. Problems arise in the interpretation of the results in the other oncotypes. In hemangiopericytomas and hemangioblastomas, laminin demonstrates a basement membrane around the vessels only; it is absent around stromal cells. In desmoplastic medulloblastomas, laminin forms a network among the tumor cells. In primary lymphomas, concentric rings of laminin are evidenced around some vessels. In discussing the results, the ultrastructural distribution of basement membranes is taken into account.

MeSH Terms
Basement Membrane/metabolism Brain Neoplasms/metabolism Histocytochemistry Humans Immunochemistry Laminin/metabolism Nerve Tissue/metabolism Reference Values Tissue Distribution
Chemicals
Laminin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Giordana M T
Germano I
Giaccone G
Mauro A
Migheli A
Schiffer D
References (38)
38 references, click to expand
  1. Clinical and pathological study of 24 cases of gliosarcoma.
    J Neurosurg. 1976 Oct;45(4):398-408 PMID: 956876
  2. Fine structure of intercellular junctions and blood vessels in medulloblastomas.
    Acta Neuropathol. 1975 Oct 27;33(1):67-78 PMID: 170780
  3. Vascular structures in brain tumors.
    Hum Pathol. 1975 Sep;6(5):611-21 PMID: 1100515
  4. CIRCUMSCRIBED SARCOMA OF THE CEREBELLUM. AN ELECTRON MICROSCOPIC STUDY.
    J Neuropathol Exp Neurol. 1964 Oct;23:706-18 PMID: 14222920
  5. The ultrastructure of medulloblastomas.
    Acta Neuropathol. 1970;16(4):271-84 PMID: 4099981
  6. The ultrastructure of acoustic nerve tumors.
    Acta Neuropathol. 1969 Jan 31;12(2):116-40 PMID: 5789731
  7. The endothelial hyperplasia of the cerebral blood vessels with brain tumors, and its sarcomatous transformation.
    Cancer. 1958 Mar-Apr;11(2):264-77 PMID: 13511345
  8. Biochemistry and metabolism of basement membranes.
    Int Rev Cytol. 1979;61:167-228 PMID: 389868
  9. Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VIII/RAg in nervous tissue after fixation.
    Histochemistry. 1984;80(2):157-63 PMID: 6325375
  10. Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein.
    Lab Invest. 1980 Mar;42(3):336-42 PMID: 6767138
  11. GFAP, F VIII/RAg, laminin, and fibronectin in gliosarcomas: an immunohistochemical study.
    Acta Neuropathol. 1984;63(2):108-16 PMID: 6428154
  12. Human and experimental reticulum cell sarcoma (microglioma) of the nervous system.
    Acta Neuropathol Suppl. 1975;Suppl 6:135-40 PMID: 1098366
  13. Laminin--a glycoprotein from basement membranes.
    J Biol Chem. 1979 Oct 10;254(19):9933-7 PMID: 114518
  14. Capillary endothelial cell cultures: phenotypic modulation by matrix components.
    J Cell Biol. 1983 Jul;97(1):153-65 PMID: 6190818
  15. Fine structure of medulloblastoma.
    Gan. 1970 Feb;61(1):17-26 PMID: 5443436
  16. Cerebellar hemangioblastoma: histogenesis of stroma cells.
    Cancer. 1973 Jun;31(6):1528-40 PMID: 4736269
  17. A comparison of the fine structure of small blood vessels in intracranial and retroperitoneal malignant lymphomas.
    Acta Neuropathol. 1974 Feb 28;27(2):93-104 PMID: 4601380
  18. Ultrastructure of cerebellar hemangioblastoma.
    Cancer. 1978 Oct;42(4):1834-50 PMID: 361212
  19. Capillary ultrastructure and the blood-brain barrier in human malignant brain tumors.
    J Neurosurg. 1970 Feb;32(2):127-44 PMID: 5411991
  20. Laminin and fibronectin distribution in normal and pathological human muscle.
    J Neurol Sci. 1983 Aug-Sep;60(3):377-82 PMID: 6355396
  21. Primary and borderline brain lymphosarcoma: a neuropathological review of nine cases.
    Acta Neuropathol Suppl. 1975;Suppl 6:107-13 PMID: 1098362
  22. Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes.
    J Cell Biol. 1982 Oct;95(1):340-4 PMID: 6216257
  23. A murine tumor producing a matrix of basement membrane.
    J Exp Med. 1977 Jan 1;145(1):204-20 PMID: 830788
  24. Basal lamina and tissue recognition in malignant mammary tumors.
    Cancer Res. 1980 May;40(5):1600-11 PMID: 7370993
  25. Distribution of endothelial and basement membrane markers in angiogenic tumors of the nervous system.
    Acta Neuropathol. 1983;62(1-2):67-72 PMID: 6419537
  26. [Electron microscopy of central nervous system hemangioblastomas and angioblastic meningiomas].
    Acta Neuropathol. 1971;19(3):184-207 PMID: 5167396
  27. Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure.
    Am J Pathol. 1974 Nov;77(2):314-46 PMID: 4614671
  28. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
    J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
  29. Production of laminin and fibronectin by Schwannoma cells: cell-protein interactions in vitro and protein localization in peripheral nerve in vivo.
    J Cell Biol. 1983 May;96(5):1218-26 PMID: 6341376
  30. Laminin is produced by early rat astrocytes in primary culture.
    J Cell Biol. 1983 Mar;96(3):920-4 PMID: 6339524
  31. Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen.
    Cell. 1980 Dec;22(3):719-26 PMID: 7460011
  32. Primary intracranial malignant lymphomas a fine structural cytochemical and CSF immunological study.
    Clin Neurol Neurosurg. 1979;81(3):173-84 PMID: 230932
  33. Fine structure of intracranial hemangiopericytoma and angiomatous meningioma.
    Cancer. 1974 Oct;34(4):1187-97 PMID: 4417339
  34. Localization of the binding site for cell attachment in the alpha1(I) chain of collagen.
    J Biol Chem. 1978 Aug 25;253(16):5642-6 PMID: 209046
  35. Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
    Histochemistry. 1982;75(3):301-7 PMID: 6183239
  36. Cerebellar capillary hemangioblastoma: its histogenesis studied by organ culture and electron microscopy.
    Cancer. 1975 Feb;35(2):326-41 PMID: 163132
  37. On the nature of the so-called monstrocellular sarcoma of the brain.
    Neurosurgery. 1980 Apr;6(4):391-7 PMID: 6993985
  38. Basement membrane surfaces and perivascular compartments in normal human brain and glial tumours. A scanning electron microscope study.
    Neuropathol Appl Neurobiol. 1983 May-Jun;9(3):181-94 PMID: 6877515
Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00688123
Published
1985-00-00
Pages
51-7
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00688123 View journal details
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