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PMID: 6325375 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Collagenase in the immunohistochemical demonstration of laminin, fibronectin and factor VIII/RAg in nervous tissue after fixation.

Histochemistry ·Vol. 80 ·No. 2 ·1984-00-00 ·Pages 157-63

Mauro A, Bertolotto A, Germano I, Giaccone G, Giordana MT, Migheli A, Schiffer D

Abstract

The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.

MeSH Terms
Antigens/analysis Brain Neoplasms/analysis,ultrastructure Factor VIII/analysis,immunology Fibronectins/analysis Fixatives Humans Immunoenzyme Techniques Laminin/analysis Microbial Collagenase Nerve Tissue/analysis,ultrastructure Pepsin A Trypsin von Willebrand Factor
Chemicals
Antigens Fibronectins Fixatives Laminin von Willebrand Factor Factor VIII Trypsin Pepsin A Microbial Collagenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mauro A
Bertolotto A
Germano I
Giaccone G
Giordana M T
Migheli A
Schiffer D
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35 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00679991
Published
1984-00-00
Pages
157-63
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00679991
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