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PMID: 4030103 Published · ppublish English Journal Article

Lymphokine activation of J774G8 cells and mouse peritoneal macrophages challenged with Toxoplasma gondii.

Infection and immunity ·Vol. 49 ·No. 3 ·1985-09-00 ·Pages 760-4

Sibley LD, Krahenbuhl JL, Weidner E

Abstract

In vitro activation of macrophage cell line J774G8 and mouse peritoneal macrophages resulted in oxygen-dependent and oxygen-independent killing of intracellular Toxoplasma gondii. Activation was characterized by oxygen-dependent killing detectable by enhanced lysosome fusion and digestion of T. gondii. The toxoplasmacidal activity of activated J774G8 cells and peritoneal macrophages was prevented by adding the oxygen intermediate scavengers catalase or superoxide dismutase during culture. Activated J774G8 cells and peritoneal macrophages also inhibited replication of those Toxoplasma organisms which survived the initial microbicidal activity. The inhibition of Toxoplasma replication was not significantly affected by exogenous catalase or superoxide dismutase. Peritoneal macrophages from Toxoplasma-immune mice showed similar microbicidal and inhibitory responses, supporting the model that activation leads to destruction of intracellular parasites by two different mechanisms.

MeSH Terms
Animals Ascites Cell Line Lymphokines/pharmacology Macrophage Activation Mice Oxygen/metabolism Toxoplasma/growth & development,immunology Toxoplasmosis/immunology
Chemicals
Lymphokines Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sibley L D
Krahenbuhl J L
Weidner E
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31 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.3.760-764.1985
Published
1985-09-00
Pages
760-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261267
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.3.760-764.1985 PMC Full Text View journal details
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