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PMID: 408513 Published · ppublish English Journal Article

Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO.

Journal of virology ·Vol. 23 ·No. 3 ·1977-09-00 ·Pages 461-6

Jarrell K, Kropinski AM

Abstract

Bacteriophage E79 was shown to interact with the lipopolysaccharide (LPS) of Pseudomonas aeruginosa strain PAO. LPS isolated from an E79-sensitive, smooth strain inactivated the phage, exhibiting a Phl50 value (concentration of LPS that caused a 50% decrease in the titer of phage during 1 h of incubation at 37 degrees C) of 0.04 microgram/ml, whereas the LPS isolated from a rough mutant derived from the wild type showed no neutralizing activity towards E79. EDTA and sodium deoxycholate were demonstrated to abolish the neutralizing capacity of the smooth LPS. One E79 receptor site was shown to be equivalent to 10(-16) g of LPS.

MeSH Terms
Adsorption Bacteriophages/growth & development,immunology,metabolism Binding Sites/drug effects Cell Wall/metabolism Deoxycholic Acid/pharmacology Edetic Acid/pharmacology Lipopolysaccharides/metabolism Mutation Neutralization Tests Polysaccharides, Bacterial/metabolism Pseudomonas aeruginosa/metabolism
Chemicals
Lipopolysaccharides Polysaccharides, Bacterial Deoxycholic Acid Edetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jarrell K
Kropinski A M
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.3.461-466.1977
Published
1977-09-00
Pages
461-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515855
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.3.461-466.1977 PMC Full Text View journal details
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