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PMID: 5499968 Published · ppublish English Journal Article

The purification and chemical composition of the lipopolysaccharide of Pseudomonas alcaligenes.

The Biochemical journal ·Vol. 120 ·No. 3 ·1970-12-00 ·Pages 559-66

Key BA, Gray GW, Wilkinson SG

Abstract

1. A method for obtaining lipopolysaccharide free from glycosaminopeptide from isolated cell walls of Pseudomonas alcaligenes is discussed. 2. About 70-75% of the lipopolysaccharide and 86-90% of the isolated lipid A have been accounted for in terms of identifiable components. 3. Hydrolysates of lipid A contain mainly inorganic phosphate, glucosamine, O-phosphorylglucosamine and fatty acids (dodecanoic acid, dodec-2-enoic acid, 3-hydroxydecanoic acid and 3-hydroxydodecanoic acid), of which the last is the main N-acylating acid of the glucosamine backbone. 4. Material corresponding to the polysaccharide moiety of the lipopolysaccharide is extensively degraded. 5. Solubilization of the lipopolysaccharide by using sodium deoxycholate appreciably affects the chemical composition of the material.

MeSH Terms
Alcaligenes/analysis Amino Acids/analysis Blood Flow Velocity Cell Wall
Chemicals
Amino Acids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Key B A
Gray G W
Wilkinson S G
References (21)
21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1200559
Published
1970-12-00
Pages
559-66
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179637
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1200559 PMC Full Text View journal details
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