Intracellular routes of apical and basolateral plasma membrane proteins to the surface of epithelial cells.
The asymmetric distribution of pumps, channels, receptors and enzymes between the apical and basolateral domains of the plasma membrane is essential for the vectorial function of transporting and secretory epithelia. Enveloped viruses that bud in a polarized fashion from the surface of epithelial cells in culture provide an excellent system to study the biogenesis of this asymmetry. Using this system, evidence has been accumulated indicating that apical and basolateral plasma membrane proteins follow the same pathway until they reach the Golgi apparatus, where they codistribute. At the level of the Golgi, or immediately after leaving this organelle, apical and basolateral glycoproteins are incorporated into separate "carrier vesicles", which transport them to the respective cell surface. Future work should establish the structural and biochemical nature of these vesicles and the molecular basis of this vectorial delivery of plasma membrane proteins in epithelial cells.
27 references, click to expand
-
Studies of renal cell function using cell culture techniques.Am J Physiol. 1980 Jan;238(1):F1-9 PMID: 6243876
-
Polarized distribution of viral envelope proteins in the plasma membrane of infected epithelial cells.Cell. 1980 May;20(1):45-54 PMID: 6248236
-
Polarized delivery of viral glycoproteins to the apical and basolateral plasma membranes of Madin-Darby canine kidney cells infected with temperature-sensitive viruses.J Cell Biol. 1985 Jan;100(1):136-51 PMID: 2981229
-
Biogenesis of epithelial cell polarity: intracellular sorting and vectorial exocytosis of an apical plasma membrane glycoprotein.Cell. 1984 Dec;39(3 Pt 2):537-46 PMID: 6509551
-
Transepithelial transport in cell culture.Proc Natl Acad Sci U S A. 1976 Apr;73(4):1212-6 PMID: 1063404
-
Intracellular transport of influenza virus hemagglutinin to the apical surface of Madin-Darby canine kidney cells.J Cell Biol. 1984 Jan;98(1):308-19 PMID: 6707094
-
Synthesis and assembly of membrane and organelle proteins.Int Rev Cytol Suppl. 1981;12:247-307 PMID: 7019120
-
Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. II. Immunological quantitation.J Cell Biol. 1983 Sep;97(3):638-43 PMID: 6411736
-
Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene.Nature. 1981 Oct 22;293(5834):620-5 PMID: 6270568
-
Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity.Proc Natl Acad Sci U S A. 1978 Oct;75(10):5071-5 PMID: 283416
-
Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK.Cell. 1984 Aug;38(1):65-77 PMID: 6088077
-
Synthesis, intracellular transport, and discharge of secretory proteins in stimulated pancreatic exocrine cells.J Cell Biol. 1971 Jul;50(1):135-58 PMID: 4327462
-
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).J Cell Biol. 1979 Jun;81(3):635-48 PMID: 222773
-
Development of cell surface polarity in the epithelial Madin-Darby canine kidney (MDCK) cell line.EMBO J. 1984 Nov;3(11):2687-94 PMID: 6391916
-
Passage of viral membrane proteins through the Golgi complex.J Mol Biol. 1981 Nov 15;152(4):663-98 PMID: 7038131
-
Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1746-50 PMID: 6262824
-
Assembly of enveloped viruses in Madin-Darby canine kidney cells: polarized budding from single attached cells and from clusters of cells in suspension.J Cell Biol. 1983 Mar;96(3):866-74 PMID: 6300140
-
Studies on the development and maintenance of epithelial cell surface polarity with monoclonal antibodies.J Cell Biol. 1984 May;98(5):1777-87 PMID: 6725399
-
Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.Cell. 1983 Aug;34(1):233-43 PMID: 6883510
-
Biogenesis of intestinal plasma membrane: posttranslational route and cleavage of sucrase-isomaltase.Proc Natl Acad Sci U S A. 1979 Oct;76(10):5183-6 PMID: 291933
-
Demonstration of protein asymmetries in the plasma membrane of cultured renal (MDCK) epithelial cells by lactoperoxidase-mediated iodination.FEBS Lett. 1979 Sep 15;105(2):201-4 PMID: 488350
-
An enzymatic assay reveals that proteins destined for the apical or basolateral domains of an epithelial cell line share the same late Golgi compartments.EMBO J. 1985 Feb;4(2):297-307 PMID: 2990898
-
Viral glycoproteins destined for apical or basolateral plasma membrane domains traverse the same Golgi apparatus during their intracellular transport in doubly infected Madin-Darby canine kidney cells.J Cell Biol. 1984 Apr;98(4):1304-19 PMID: 6325468
-
Polarized monolayers formed by epithelial cells on a permeable and translucent support.J Cell Biol. 1978 Jun;77(3):853-80 PMID: 567227
-
Intracellular aspects of the process of protein synthesis.Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
-
Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. I. Morphological evidence.J Cell Biol. 1983 Sep;97(3):627-37 PMID: 6885914
-
Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells.Cell. 1982 Oct;30(3):753-62 PMID: 6291783
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
