Home LiteratureArticle Details

PMID: 4092697 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA intercalators induce specific release of HMG 14, HMG 17 and other DNA-binding proteins from chicken erythrocyte chromatin.

The EMBO journal ·Vol. 4 ·No. 13B ·1985-12-30 ·Pages 3867-72

Schröter H, Maier G, Ponstingl H, Nordheim A

Abstract

Chicken erythrocyte nuclei were incubated with DNA intercalating agents in order to isolate from chromatin specific DNA-binding proteins whose binding specificity may be determined by DNA secondary and/or tertiary structure. The intercalating agents ethidium bromide (EtBr) and propidium iodide induce the specific release of high mobility group proteins HMG 14 and HMG 17 under low ionic strength conditions. Chloroquine (CQ) intercalation also results in the selective liberation of HMG 14 and HMG 17, but, in addition, selectively releases other nuclear proteins (including histone H1A) in a pH- and ionic strength-dependent fashion. The use of this new 'elutive intercalation' technique for the isolation and purification of 'sequence-specific' and 'helix-specific' DNA-binding proteins is suggested.

MeSH Terms

Animals Chickens Chromatin/drug effects,metabolism DNA-Binding Proteins/blood,isolation & purification Erythrocytes/metabolism Ethidium/pharmacology High Mobility Group Proteins/blood,isolation & purification Intercalating Agents Molecular Weight Phenanthridines/pharmacology Propidium/pharmacology

Chemicals

Chromatin DNA-Binding Proteins High Mobility Group Proteins Intercalating Agents Phenanthridines Propidium Ethidium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schröter H

Maier G

Ponstingl H

Nordheim A

References (26)

26 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
Gene. 1985;33(1):103-19 PMID: 2985470
Relaxation of chromatin structure by ethidium bromide binding: determined by viscometry and histone dissociation studies.
Biochemistry. 1976 Nov 2;15(22):4803-9 PMID: 990244
Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810-4 PMID: 268631
Chomosomal HMG proteins occur in three eukaryotic kingdoms.
Biochem Biophys Res Commun. 1978 May 15;82(1):129-35 PMID: 352357
Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 PMID: 284387
The complete amino-acid sequence of a trout-testis non-histone protein, H6, localized in a subset of nucleosomes and its similarity to calf-thymus non-histone proteins HMG-14 and HMG-17.
Eur J Biochem. 1979 Mar 15;95(1):193-202 PMID: 456349
The primary structure of non-histone chromosomal protein HMG17 from chicken erythrocyte nuclei.
FEBS Lett. 1980 Apr 7;112(2):207-10 PMID: 7371857
The isolation, characterization and partial sequences of the chicken erythrocyte non-histone chromosomal proteins HMG14 and HMG17. Comparison with the homologous calf thymus proteins.
Biochem J. 1980 Feb 1;185(2):383-6 PMID: 7396821
The release of high mobility group protein H6 and protamine gene sequences upon selective DNase I degradation of trout testis chromatin.
Nucleic Acids Res. 1980 Jul 11;8(13):2859-69 PMID: 6253894
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.
Nucleic Acids Res. 1980 Sep 11;8(17):3757-78 PMID: 6449690
Identification of the phenylthiohydantoin derivatives of amino acids by high pressure liquid chromatography, using a ternary, isocratic solvent system.
Hoppe Seylers Z Physiol Chem. 1980 Dec;361(12):1829-34 PMID: 7461608
Active chromatin.
Nature. 1982 May 27;297(5864):289-95 PMID: 6210847
The three-dimensional structure of DNA.
Annu Rev Biochem. 1982;51:395-427 PMID: 7051961
Chromatin.
Annu Rev Biochem. 1982;51:89-121 PMID: 6287925
The binding sites for large and small high-mobility-group (HMG) proteins. Studies on HMG-nucleosome interactions in vitro.
Eur J Biochem. 1982 Oct;127(2):429-36 PMID: 6216108
Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7729-33 PMID: 6296848
Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
Methods Enzymol. 1983;91:227-36 PMID: 6855576
High-sensitivity sequencing with a gas-phase sequenator.
Methods Enzymol. 1983;91:399-413 PMID: 6855591
DNA supercoiling and its effects on DNA structure and function.
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:85-91 PMID: 6345067
Nonhistone nuclear high mobility group proteins 14 and 17 stabilize nucleosome core particles.
J Biol Chem. 1983 Nov 10;258(21):13221-9 PMID: 6226664
DNA conformation at the 5' end of the chicken adult beta-globin gene.
Cell. 1983 Dec;35(2 Pt 1):467-77 PMID: 6317190
Kinked DNA in crystalline complex with EcoRI endonuclease.
Nature. 1984 May 24-30;309(5966):327-31 PMID: 6328307
Protein-DNA recognition.
Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
Structure of the nucleosome core particle at 7 A resolution.
Nature. 1984 Oct 11-17;311(5986):532-7 PMID: 6482966
Ethidium bromide as a cooperative effector of a DNA structure.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3805-9 PMID: 4509343

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1985-12-30

Pages

3867-72

Language

English

Region

England

NLM ID

8208664

PMCID

PMC554742

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

DNA intercalators induce specific release of HMG 14, HMG 17 and other DNA-binding proteins from chicken erythrocyte chromatin.

Contact

WeChat Official Account