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PMID: 410003 Published · ppublish English Journal Article

Oligonucleotide mapping of non-radioactive virus and messenger RNAs.

Nucleic acids research ·Vol. 4 ·No. 9 ·1977-09-00 ·Pages 2975-96

Frisby D

Abstract

A modification of the known method for obtaining radioactive fingerprints from non-radioactive nucleic acids by labelling a digest with 5'-hydroxyl polynucleotide kinase and [gamma-32P]-ATP has been applied to RNase T1 digests from various high molecular weight virus RNAs and to ovalbumin mRNA. Fractionation of the resultant [32P]-labelled T1 RNase digests by two-dimensional polyacrylamide electrophoresis demonstrates that in the case of virus RNAs, the fingerprints thus obtained are very similar to those derived from uniformly labelled RNAs. The value of this technique is that it requires only 1-5 microgram of purified virus RNA and at least three orders of magnitude less radioactivity than is routinely employed in preparing uniformly labelled RNA.

MeSH Terms
Adenosine Triphosphate Alkaline Phosphatase Electrophoresis, Polyacrylamide Gel Isotope Labeling/methods Oligoribonucleotides/analysis Phosphorus Radioisotopes RNA, Messenger RNA, Viral Ribonuclease T1 Species Specificity
Chemicals
Oligoribonucleotides Phosphorus Radioisotopes RNA, Messenger RNA, Viral Adenosine Triphosphate Ribonuclease T1 Alkaline Phosphatase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Frisby D
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.9.2975
Published
1977-09-00
Pages
2975-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342628
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.9.2975 PMC Full Text View journal details
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