Home LiteratureArticle Details
PMID: 410030 Published · ppublish English Comparative Study Journal Article

Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 9 ·1977-09-00 ·Pages 4002-6

Terhorst C, Robb R, Jones C, Strominger JL

Abstract

The papain-solubilized fragment of the heavy chain of HLA-B7, which is the NH2-terminal part of the whole polypeptide chain, can be divided into three regions by mild acid and cyanogen bromide cleavages. The first 100 amino acids terminating in a methionine residue contain the carbohydrate moiety; this segment is followed by two others of molecular weights 9,999 and 13,000, each containing an intrachain disulfide bridge. The two intrachain disulfide bridges are separated by a stretch of amino acids containing an acid-labile aspartyl-prolHLA-2, A28, and AW25 contain this acid-labile peptide bond in their larger subunit. Sequencing from the acid cleavage site of HLA-7 through the third half-cystine revealed consideralbe homology with amino acid sequences around a half-cystine in immunoglobulin variable regions.

MeSH Terms
Amino Acid Sequence Cell Line Cell Membrane/analysis Disulfides/analysis HLA Antigens/isolation & purification Immunoglobulin Heavy Chains/isolation & purification Macromolecular Substances Molecular Weight Peptide Fragments/analysis
Chemicals
Disulfides HLA Antigens Immunoglobulin Heavy Chains Macromolecular Substances Peptide Fragments
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Terhorst C
Robb R
Jones C
Strominger J L
References (33)
33 references, click to expand
  1. Nonenzymatic cleavage of peptide bonds: the methionine residues in bovine pancreatic ribonuclease.
    J Biol Chem. 1962 Jun;237:1856-60 PMID: 13902203
  2. Partial amino-acid sequences of human and rabbit C-reactive proteins: homology with immunoglobulins and histocompatibility antigens.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1214-8 PMID: 403526
  3. Functional specificity of thymus- dependent lymphocytes.
    Science. 1977 Mar 25;195(4284):1293-300 PMID: 320663
  4. Glycoprotein staining following electrophoresis on acrylamide gels.
    Anal Biochem. 1969 Jul;30(1):148-52 PMID: 4183001
  5. The genetic control of immunoglobulin synthesis.
    Annu Rev Genet. 1972;6:1-46 PMID: 4124595
  6. The immunoglobulin-like structure of human histocompatibility antigens.
    Transplant Rev. 1974;21(0):126-43 PMID: 4139785
  7. Detergent-solubilised H-2 alloantigen is associated with a small molecular weight polypeptide.
    Nature. 1974 Jun 21;249(459):764-5 PMID: 4134396
  8. The small subunit of HL-A antigens is beta 2-microglobulin.
    J Exp Med. 1973 Dec 1;138(6):1608-12 PMID: 4128442
  9. Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):35-9 PMID: 4129801
  10. Immunological identity of the small subunit of HL-A antigens and beta2-microglobulin and its turnover on the cell membrane.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2123-7 PMID: 4134761
  11. Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations.
    Biochem Biophys Res Commun. 1970 Sep 10;40(5):1173-8 PMID: 4100801
  12. Amino-acid sequence of alpha chains of human haptoglobins.
    Nature. 1968 May 25;218(5143):736-41 PMID: 4172407
  13. Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids: evidence for synteny between the HL-A, PGM3, ME1, and IPO-B loci.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):962-5 PMID: 4362641
  14. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization.
    Adv Immunol. 1975;20:125-95 PMID: 47219
  15. Partial purification of detergent-soluble HL-A antigen and its cleavage by papain.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1539-43 PMID: 4208551
  16. Rapid purification of detergent-solubilized HLA antigen by affinity chromatography employing anti-beta2-microglobulin serum.
    J Biol Chem. 1976 Sep 10;251(17):5427-8 PMID: 60339
  17. The major histocompatibility complex--genetics and biology. (First of three parts).
    N Engl J Med. 1976 Oct 7;295(15):806-13 PMID: 60701
  18. Role of the murine major histocompatibility complex in the specificity of in vitro T-cell-mediated lympholysis against chemically-modified autologous lymphocytes.
    Transplant Rev. 1976;29:222-46 PMID: 58458
  19. H-2 compatability requirement for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. Different cytotoxic T-cell specificities are associated with structures coded for in H-2K or H-2D;.
    J Exp Med. 1975 Jun 1;141(6):1427-36 PMID: 47901
  20. The beta2-microglobulin gene is on chromosome 15 and not in the HL-A region.
    Nature. 1975 Mar 20;254(5497):267-9 PMID: 46595
  21. 2 -Microglobulin--a free immunoglobulin domain.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697-701 PMID: 4558655
  22. Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2863-5 PMID: 4517941
  23. The minor proteins in tomato bushy stunt and turnip crinkle viruses.
    Virology. 1974 Jun;59(2):509-15 PMID: 4833541
  24. Papain-solubilized HL-A antigens. Chromatographic and electrophoretic studies of the two subunits from different specificities.
    J Biol Chem. 1974 May 10;249(9):2828-32 PMID: 4828322
  25. Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin.
    Biochem Biophys Res Commun. 1972 May 26;47(4):923-30 PMID: 5026302
  26. Initiation of protein synthesis at an unusual position in an immunoglobulin gene?
    Science. 1972 Jan 14;175(4018):187-9 PMID: 5008437
  27. Hydrolysis of proteins.
    Adv Protein Chem. 1965;20:37-107 PMID: 5334827
  28. Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2481-5 PMID: 781677
  29. Evolutionary relationship between immunoglobulins and transplantation antigens.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1612-6 PMID: 1055432
  30. Immunological and chemical purity of papain-solubilized HL-A antigens.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1594-8 PMID: 1055430
  31. Similarity of three-dimensional structure between the immunoglobulin domain and the copper, zinc superoxide dismutase subunit.
    J Mol Biol. 1976 Apr 5;102(2):221-35 PMID: 1271464
  32. Amination of carboxyl-terminal homoserine peptides as an aid in peptide separation.
    Anal Biochem. 1975 Dec;69(2):583-9 PMID: 1217723
  33. Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI 4265.
    J Biol Chem. 1975 Jun 25;250(12):4512-9 PMID: 1141219
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.9.4002
Published
1977-09-00
Pages
4002-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431819
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.9.4002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2