Biochemical differentiation in reaggregating brain cell culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1858-61

Seeds NW

Abstract

Dissociated cells from embryonic mouse brain reassociate in rotation culture to form aggregates. During cell culture the specific activities of choline acetyl-transferase (EC 2.3.1.6), acetylcholinesterase (EC 3.1.1.7), and glutamate decarboxylase (EC 4.1.1.15) in the aggregates increase up to twenty-fold, a phenomenon that approximates some of the biochemical events in the development of the mouse brain.

MeSH Terms

Acetylcholinesterase/analysis Acyltransferases/analysis Age Factors Animals Brain/cytology,embryology,enzymology Carbon Isotopes Carboxy-Lyases/analysis Cell Aggregation Cell Line Cells, Cultured/enzymology Choline Clone Cells Cricetinae Female Glutamates Histocytochemistry Mice Neuroblastoma/enzymology Ovary/enzymology Staining and Labeling Time Factors

Chemicals

Carbon Isotopes Glutamates Acyltransferases Acetylcholinesterase Carboxy-Lyases Choline

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Seeds N W

References (17)

17 references, click to expand

A simplified radiochemical assay for choline acetyltransferase.
J Neurochem. 1967 Oct;14(10):977-85 PMID: 6053726
The formation of synaptic junctions in developing rat brain: a quantitative electron microscopic study.
Brain Res. 1967 Dec;6(4):716-27 PMID: 4169903
Differentiated rat glial cell strain in tissue culture.
Science. 1968 Jul 26;161(3839):370-1 PMID: 4873531
Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81 PMID: 5244736
L-glutamic acid decarboxylase: a new type in glial cells and human brain gliomas.
Science. 1970 May 1;168(3931):598-9 PMID: 5436595
Histogenesis of fetal mouse isocortex and hippocampus in reaggregating cell cultures.
Dev Biol. 1970 Aug;22(4):563-83 PMID: 5460489
Regulation of axon formation by clonal lines of a neural tumor.
Proc Natl Acad Sci U S A. 1970 May;66(1):160-7 PMID: 5273894
Regulation of acetylcholinesterase in neuroblastoma cells.
Proc Natl Acad Sci U S A. 1970 Oct;67(2):786-92 PMID: 5289021
The determination of cholinesterase activity in whole brains of developing rats.
Science. 1951 Apr 6;113(2936):382-3 PMID: 14817313
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
gamma Aminobutyric acid content and glutamic decarboxylase activity in developing mouse brain.
Proc Soc Exp Biol Med. 1951 Dec;78(3):799-803 PMID: 14912036
Two selective inhibitors of cholinesterase.
Biochem J. 1953 Jul;54(4):695-700 PMID: 13058976
Choline acetylase in the developing nervous system of the rabbit and guinea-pig.
J Physiol. 1956 Sep 27;133(3):566-70 PMID: 13368105
Quantitative histochemical changes during the development of the rat cerebral cortex.
J Neurochem. 1956 Dec;1(2):173-80 PMID: 13398834
Quantitative histochemical studies of the morphogenesis of the cerebellum. I. Total lipid and four enzymes.
J Neurochem. 1961 Nov;8:81-95 PMID: 14492878
Some properties of L-glutamic decarboxylase in mouse brain.
Biochem Pharmacol. 1963 Feb;12:113-34 PMID: 13974291
CHANGES OF MITOCHONDRIAL GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND 6-PHOSPHOGLUCONATE DEHYDROGENASE DURING BRAIN DEVELOPMENT.
Biochim Biophys Acta. 1965 May 18;99:367-9 PMID: 14336074

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.8.1858

Published

1971-08-00

Pages

1858-61

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389308

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.8.1858 PMC Full Text View journal details

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