Regulation of acetylcholinesterase in neuroblastoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 2 ·1970-10-00 ·Pages 786-92

Blume A, Gilbert F, Wilson S, Farber J, Rosenberg R, Nirenberg M

Abstract

The specific activity of mouse neuroblastoma acetylcholinesterase (EC 3.1.1.7) increased 25-fold when the rate of cell division was restricted. The results show that acetylcholinesterase activity is regulated in neuroblastoma cells and that the regulatory mechanism is inversely related to the rate of cell division. Under the same conditions the specific activity of catechol-O-methyl transferase (EC 2.1.1.6) did not change significantly.

MeSH Terms

Acetylcholinesterase/metabolism Animals Blood Carbon Isotopes Cell Differentiation Cell Division/drug effects Cell Transformation, Neoplastic/drug effects Clone Cells/enzymology Culture Media Cycloheximide/pharmacology Enzyme Activation Esterases/antagonists & inhibitors Mice Neoplasm Proteins/biosynthesis Neoplasms, Experimental/enzymology Neuroblastoma/enzymology Thymidine/pharmacology Transferases/metabolism Trypsin/pharmacology

Chemicals

Carbon Isotopes Culture Media Neoplasm Proteins Cycloheximide Transferases Esterases Acetylcholinesterase Trypsin Thymidine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Blume A

Gilbert F

Wilson S

Farber J

Rosenberg R

Nirenberg M

References (11)

11 references, click to expand

Acetylcholine sensitivity and distribution on mouse neuroblastoma cells.
Science. 1970 Feb 27;167(3922):1253-5 PMID: 4312553
A direct radioisotopic assay for acetylcholinesterase.
Anal Biochem. 1966 Jul;16(1):59-64 PMID: 5966131
Establishment of functional clonal lines of neurons from mouse neuroblastoma.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):311-5 PMID: 5263016
Isolation of microtubule protein from cultured mouse neuroblastoma cells.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):129-36 PMID: 5263744
Neuronal tumor cells with excitable membranes grown in vitro.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1004-10 PMID: 5264132
Regulation of axon formation by clonal lines of a neural tumor.
Proc Natl Acad Sci U S A. 1970 May;66(1):160-7 PMID: 5273894
Two selective inhibitors of cholinesterase.
Biochem J. 1953 Jul;54(4):695-700 PMID: 13058976
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
The effects of some acetylcholine derivatives on the cholinesterases of chick embryo intestine cultured in vitro.
J Pharmacol Exp Ther. 1957 Jul;120(3):285-90 PMID: 13476351
Counting actively metabolizing tissue cultured cells.
Exp Cell Res. 1957 Oct;13(2):341-7 PMID: 13480301
EFFECTS OF SPINAL CORD AND SUBSTRATE ON ACETYLCHOLINESTERASE IN CHICK EMBRYONIC SKELETAL MUSCLE.
Dev Biol. 1965 Feb;11:136-53 PMID: 14300091

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.2.786

Published

1970-10-00

Pages

786-92

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283274

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.2.786 PMC Full Text View journal details

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Regulation of acetylcholinesterase in neuroblastoma cells.

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