Mechanism of action of ribonuclease H isolated from avian myeloblastosis virus and Escherichia coli.
Purified preparations of RNA-dependent DNA polymerase isolated from avain myeloblastosis virus contain RNase H activity. Labeled ribohomopolymers are degraded in the presence of their complementary deoxyribopolymer, except [(3)H]poly(U).poly(dA). The degradation products formed from [(3)H]poly(A).poly(dT) were identified as oligonucleotides containing 3'-hydroxyl and 5'-phosphate termini, while AMP was not detected. The nuclease has been characterized as a processive exonuclease that requires ends of poly(A) chains for activity. Exonucleolytic attack occurs in both 5' to 3' and 3' to 5' directions.RNase H has also been purified from E. coli. This nuclease degrades all homoribopolymers tested in the presence of their complementary deoxyribopolymers to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. E. coli RNase H has been characterized as an endonuclease.
15 references, click to expand
-
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 PMID: 5323016
-
The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II.J Biol Chem. 1968 Mar 10;243(5):913-22 PMID: 4867942
-
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.J Biol Chem. 1969 Jun 10;244(11):2996-3008 PMID: 4890762
-
Analysis of the ribonucleic acid of murine leukemia virus.J Virol. 1969 Oct;4(4):454-9 PMID: 4309879
-
RNA-dependent DNA polymerase in virions of RNA tumour viruses.Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300
-
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
-
Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids.Nat New Biol. 1971 Dec 22;234(51):240-3 PMID: 4331605
-
Deoxyadenosine diphosphate as a substrate and inhibitor of polynucleotide phosphorylase of Micrococcus luteus. I. Deoxyadenosine diphosphate as a substrate for polymerization and the exchange reaction with inorganic 32 P.J Biol Chem. 1971 Dec 25;246(24):7486-96 PMID: 5316336
-
Isolation and characterization of a protein that stimulates DNA synthesis from avian myeloblastosis virus.Proc Natl Acad Sci U S A. 1972 Aug;69(8):2331-5 PMID: 4340754
-
Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
-
Purification and properties of bacteriophage T4-induced RNA ligase.Proc Natl Acad Sci U S A. 1972 Oct;69(10):3009-13 PMID: 4342972
-
Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360-4 PMID: 4343966
-
Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase.J Biol Chem. 1972 Nov 25;247(22):7282-7 PMID: 4344646
-
THE ROLE OF DEOXYRIBONUCLEIC ACID IN THE SYNTHESIS OF ROUS SARCOMA VIRUS.Virology. 1964 Apr;22:462-8 PMID: 14166107
-
EFFECTS OF ACTINOMYCIN D AND OF MITOMYCIN C ON THE DEVELOPMENT OF ROUS SARCOMA VIRUS.Virology. 1964 Aug;23:511-9 PMID: 14204704
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