Home LiteratureArticle Details
PMID: 4120434 Published · ppublish English Journal Article

The distribution of surface antigens during fractionation of mouse liver plasma membranes.

The Biochemical journal ·Vol. 130 ·No. 1 ·1972-11-00 ·Pages 271-80

Gurd JW, Evans WH, Perkins HR

Abstract

1. Antiserum to purified mouse liver plasma membranes was prepared and the partially purified gamma-globulin antibody fraction was iodinated with (125)I. The reaction of the (125)I-labelled gamma-globulin antibody with isolated mouse liver plasma membranes was studied. 2. The gammaglobulin antibody bound specifically to mouse liver plasma membranes and there was little reaction with mouse liver intracellular membranes or with surface-membrane fractions from either rat liver or pig lymphocytes. 3. ;Light' and ;heavy' mouse liver plasma-membrane subfractions bound similar amounts of gamma-globulin antibody, and this is consistent with a surface origin for the light fraction. 5. Plasma membranes were fractionated by sequential extraction with 50mm-NaHCO(3)-Na(2)CO(3) buffer, pH10.2, containing 10mm-EDTA and aq. 33% (v/v) pyridine. The alkali-soluble and -insoluble fractions and the pyridine-soluble and -insoluble fractions all reacted with the antiserum, and the cross-reactivity among the various fractions and with the total plasma membranes was investigated. 5. The results are discussed in terms of the arrangement of the antigenic determinants within the membrane.

MeSH Terms
Animals Antigen-Antibody Reactions Antigens/isolation & purification Cell Membrane/immunology Centrifugation, Zonal Immune Sera Iodine Isotopes Liver/immunology Lymphocytes/immunology Mice Protein Binding Rabbits/immunology Subcellular Fractions/immunology gamma-Globulins
Chemicals
Antigens Immune Sera Iodine Isotopes gamma-Globulins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gurd J W
Evans W H
Perkins H R
References (37)
37 references, click to expand
  1. An approach to the mapping of antigens on the cell surface.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):886-93 PMID: 5243930
  2. The fluid mosaic model of the structure of cell membranes.
    Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
  3. Organizational difference of cell surface "hematoside" in normal and virally transformed cells.
    Biochem Biophys Res Commun. 1968 Nov 25;33(4):563-8 PMID: 4301484
  4. Fractionation of liver plasma membranes prepared by zonal centrifugation.
    Biochem J. 1970 Mar;116(5):833-42 PMID: 4315049
  5. Sulfanilic acid diazonium salt: a label for the outside of the human erythrocyte membrane.
    Biochim Biophys Acta. 1969 Jun 3;183(1):65-78 PMID: 4183114
  6. Biosynthesis of liver membranes. Incorporation of ( 3 H)leucine into proteins and of ( 14 C)glucosamine into proteins and lipids of liver microsomal and plasma-membrane fractions.
    Biochem J. 1971 Nov;125(2):615-24 PMID: 5144762
  7. A FLUORESCENT LABEL FOR THE OUTER COMPONENTS OF THE PLASMA MEMBRANE.
    Biochim Biophys Acta. 1964 Sep 25;88:390-9 PMID: 14253225
  8. Studies on plasma membranes. XVI. Tissue specific antigens in the liver cell surface.
    Exp Cell Res. 1972 Mar;71(1):97-105 PMID: 4112659
  9. Alterations of Forssman-antigenic reactivity and of monosaccharide composition in plasma membrane from polyoma-transformed hamster cells.
    Biochim Biophys Acta. 1971 Aug 13;241(2):403-11 PMID: 4334144
  10. Immunological analysis of Mycoplasma membranes.
    J Bacteriol. 1969 Oct;100(1):187-94 PMID: 4981055
  11. Efficient trace-labelling of proteins with iodine.
    Nature. 1958 Jul 5;182(4627):53 PMID: 13566175
  12. GLOBULAR LIPID MICELLES AND CELL MEMBRANES.
    J Theor Biol. 1964 Sep;7:360-73 PMID: 14212282
  13. A major protein which spans the human erythrocyte membrane.
    J Mol Biol. 1971 Jul 28;59(2):351-7 PMID: 5566420
  14. Comparison of the effects of various detergents on antigen-antibody interaction.
    FEBS Lett. 1972 May 1;22(2):210-212 PMID: 11946599
  15. The site of diphosphoinositide synthesis in rat liver.
    Biochem Biophys Res Commun. 1965 Nov 22;21(4):333-8 PMID: 4286175
  16. The immunological activity of some of the chymotryptic peptides of sperm-whale myoglobin.
    Biochem J. 1965 Mar;94(3):545-56 PMID: 16749070
  17. Subfractionation of rat liver plasma membranes.
    FEBS Lett. 1969 Jun;3(4):237-241 PMID: 11947017
  18. Gel electrophoresis patterns of the proteins of organelles isolated from bovine liver.
    Biochim Biophys Acta. 1970 Apr 21;203(2):283-90 PMID: 5441387
  19. A difference in the architecture of the surface membrane of normal and virally transformed cells.
    Proc Natl Acad Sci U S A. 1969 Mar;62(3):994-1001 PMID: 4308100
  20. The localization of spectrin on the inner surface of human red blood cell membranes by ferritin-conjugated antibodies.
    J Cell Biol. 1971 Oct;51(1):265-72 PMID: 5000071
  21. The morphology of the polysaccharide coat of mammalian cells.
    Biochim Biophys Acta. 1972 Feb 14;265(1):85-113 PMID: 4111356
  22. Glycoproteins of mouse liver smooth microsomal and plasma membrane fractions.
    Biochim Biophys Acta. 1970 Sep 15;211(3):578-81 PMID: 4318591
  23. Purification and properties of nucleotide pyrophosphatase from rat liver plasma membranes.
    FEBS Lett. 1972 Mar;21(1):95-98 PMID: 11946484
  24. The dispositions of proteins in the plasma membranes of animal cells: analytical approaches using controlled peptidolysis and protein labels.
    Biochim Biophys Acta. 1972 Feb 14;265(1):61-83 PMID: 4552306
  25. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  26. Studies on the distribution of some histocompatibility antigens in mouse liver plasma membrane and microsomal fractions.
    Immunology. 1970 Nov;19(5):735-41 PMID: 4320829
  27. Exposed protein on the intact human erythrocyte.
    Biochemistry. 1971 May 11;10(10):1766-71 PMID: 5563761
  28. Two locations of the lac permease sulphydryl in the membrane of E. coli.
    FEBS Lett. 1969 Nov 12;5(3):173-176 PMID: 11947269
  29. A study of the structural integrity of spin-labelled proteins in some fractions of human erythrocyte ghosts.
    Biochim Biophys Acta. 1970;219(1):73-80 PMID: 4319694
  30. WTwo protein fractions obtained from hepatic plasma membranes. Studies of their composition and differential turnover.
    Biochim Biophys Acta. 1970 Dec 1;219(2):349-60 PMID: 5515421
  31. The low polarity of many membrane proteins.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):930-2 PMID: 4502942
  32. Preparation and characterization of the plasma membrane of pig lymphocytes.
    Biochem J. 1970 Nov;120(1):133-43 PMID: 4321928
  33. Selective solubilization of a protein component of the red cell membrane.
    Science. 1968 Jan 12;159(3811):203-4 PMID: 5634911
  34. Chemical characterization of the proteins and glycoproteins of mouse liver plasma membranes solubilized by sequential extraction with aqueous and organic solvents.
    Biochem J. 1972 Feb;126(3):459-66 PMID: 5075260
  35. Permeability of membrane junctions.
    Ann N Y Acad Sci. 1966 Jul 14;137(2):441-72 PMID: 5229810
  36. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
    Biochem J. 1961 Nov;81:384-92 PMID: 13860975
  37. Immunochemical characterization of enzyme activities in liver plasma membranes of the rat.
    Exp Cell Res. 1971 May;66(1):104-12 PMID: 4103443
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1300271
Published
1972-11-00
Pages
271-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1174324
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1300271 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2