Home LiteratureArticle Details
PMID: 4127028 Published · ppublish English Comparative Study Journal Article

Lack of serological relationship among DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken cells.

Journal of virology ·Vol. 12 ·No. 3 ·1973-09-00 ·Pages 440-8

Mizutani S, Temin HM

Abstract

Antibodies against a large and a small DNA polymerase isolated from chicken embryos and against avian myeloblastosis virus DNA polymerase were used to study the serological relationships of the DNA polymerase activities of three avian systems with RNA and a DNA polymerase-avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and a fraction from uninfected chicken cells. The DNA polymerase activity of disrupted virions of all avian leukosis-sarcoma viruses tested was neutralized to the same extent by antibody against avian myeloblastosis virus DNA polymerase and was not neutralized by the antibodies against chicken cellular DNA polymerases. The viruses tested included induced leukosis viruses and Rous-associated virus-O. The DNA polymerase activity of disrupted virions of all of the reticuloendotheliosis viruses was not neutralized by any of the antibodies. The chicken endogenous RNA-directed DNA polymerase activity was neutralized partially or completely, in different experiments, by antibody against the small DNA polymerase isolated from chicken embryos, but was not neutralized by the other two antibodies.

MeSH Terms
Alpharetrovirus/classification,enzymology Animals Antigens, Viral Birds Cells, Cultured/enzymology,immunology Centrifugation, Density Gradient Chick Embryo Chromatography Immunoglobulin G Neutralization Tests Quail RNA-Directed DNA Polymerase/isolation & purification,metabolism Rats Thymine Nucleotides/metabolism Tritium Virus Cultivation
Chemicals
Antigens, Viral Immunoglobulin G Thymine Nucleotides Tritium RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mizutani S
Temin H M
References (32)
32 references, click to expand
  1. Electron microscopic observations on RE virus (strain T) that induces reticuloendotheliosis in turkeys, chickens, and Japanese quail.
    J Natl Cancer Inst. 1966 Dec;37(6):709-29 PMID: 4289066
  2. Biological studies with RE virus (strain T) that induces reticuloendotheliosis in turkeys, chickens, and Japanese quail.
    J Natl Cancer Inst. 1966 Dec;37(6):731-43 PMID: 4289067
  3. Cultivation of a filterable agent associated with Marek's disease.
    J Natl Cancer Inst. 1969 Jul;43(1):203-12 PMID: 4307703
  4. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses.
    Virology. 1970 Jan;40(1):118-34 PMID: 4312371
  5. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
    Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
  6. Recovery of a new virus from apparently normal chick cells by infection with avian tumor viruses.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1797-803 PMID: 4321346
  7. An avian leukosis virus related to RSV(O): properties and evidence for helper activity.
    Virology. 1971 Jan;43(1):223-34 PMID: 5101017
  8. Noninfectious RSV deficient in DNA polymerase.
    Virology. 1971 Jan;43(1):313-6 PMID: 5101018
  9. Separation of murine cellular and murine leukaemia virus DNA polymerases.
    Nat New Biol. 1971 Jun 9;231(23):163-7 PMID: 5281193
  10. Comparison of Rous sarcoma virus-specific deoxyribonucleic acid polymerases in virions of Rous sarcoma virus and in Rous sarcoma virus-infected chicken cells.
    J Virol. 1971 May;7(5):625-34 PMID: 4326752
  11. Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520-4 PMID: 4327005
  12. Deoxyribonucleic acid polymerase with rat liver ribosomes and smooth membranes. Purification and properties of the enzymes.
    Biochemistry. 1971 May 25;10(11):1981-92 PMID: 4327392
  13. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.
    Biochem Biophys Res Commun. 1971 Aug 20;44(4):879-85 PMID: 5166448
  14. Ribonuclease-sensitive deoxyribonucleic acid polymerase activity in uninfected rat cells and rat cells infected with Rous sarcoma virus.
    J Virol. 1971 Nov;8(5):630-42 PMID: 4332135
  15. Separation of reticuloendotheliosis virus from avian tumor viruses.
    J Virol. 1971 Nov;8(5):813-5 PMID: 4332147
  16. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens.
    Virology. 1971 Dec;46(3):920-38 PMID: 4332981
  17. Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses.
    J Virol. 1972 Jan;9(1):110-5 PMID: 4333537
  18. Avian reticuloendotheliosis virus (strain T): V. DNA polymerase.
    Virology. 1972 Jan;47(1):251-4 PMID: 4333573
  19. Antigenic relationships in mammalian DNA polymerase.
    Science. 1972 Mar 10;175(4026):1116-7 PMID: 4110484
  20. Avian reticuloendotheliosis virus (strain T). II. Biochemical and biophysical properties.
    Avian Dis. 1971 Oct-Dec;15(4):850-62 PMID: 4334077
  21. Avian reticuloendotheliosis virus (strain T). 3. Ultrastructure studies.
    Avian Dis. 1971 Oct-Dec;15(4):863-73 PMID: 4110517
  22. Duck infectious anemia virus associated with Plasmodium lophurae.
    Exp Parasitol. 1972 Feb;31(1):29-38 PMID: 4622046
  23. Deficiency of viral ribonucleic acid-dependent deoxyribonucleic acid polymerase in noninfectious virus-like particles released from murine sarcoma virus-transformed hamster cells.
    J Virol. 1972 Mar;9(3):488-93 PMID: 4335663
  24. Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1550-4 PMID: 4338597
  25. Defective mouse sarcoma virus deficient in DNA polymerase activity.
    J Gen Virol. 1972 Aug;16(2):223-6 PMID: 4342041
  26. Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. II. Comparison of avian deoxyribonucleic acid polymerases.
    J Virol. 1972 Nov;10(5):959-64 PMID: 4117966
  27. Isolation of leukosis-type virus from pheasant embryo cells: possible presence of viral genes in cells.
    Virology. 1973 Jan;51(1):247-51 PMID: 4346298
  28. RNA-directed DNA synthesis and RNA tumor viruses.
    Adv Virus Res. 1972;17:129-86 PMID: 4348509
  29. Early DNA-RNA complex from the endogenous RNA-directed DNA polymerase activity of uninfected chicken embryos.
    Nat New Biol. 1973 Apr 18;242(120):206-8 PMID: 4122152
  30. A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses.
    Virology. 1973 Jun;53(2):287-99 PMID: 4351341
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. A new virus of ducks interfering with development of malaria parasite (Plasmodium lophurae).
    Proc Soc Exp Biol Med. 1959 Jul;101(3):578-82 PMID: 13675324
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.3.440-448.1973
Published
1973-09-00
Pages
440-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356649
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.3.440-448.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2