Home LiteratureArticle Details
PMID: 4127032 Published · ppublish English Journal Article

Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells.

Journal of virology ·Vol. 12 ·No. 3 ·1973-09-00 ·Pages 564-9

Stephenson JR, Aaronson SA

Abstract

Highly sensitive and specific radioimmunoassays are described for quantitation of the intraspecies determinants of several mammalian C-type viral group-specific (gs) antigens. An interspecies (gs-3) immunoassay has been developed which has both the broad reactivity and great sensitivity necessary for detection of C-type viruses where intraspecies gs assays are not available. By using these immunoassays, the expression of endogenous virus-specified gs antigens in mammalian cells of different species has been studied. Whereas mouse gs antigen was clearly detectable in tissue culture cells of several mouse strains, the respective gs antigens of rat, cat, Chinese hamster, woolly monkey, and gibbon ape were not detectable in cells of those species, using assays of comparable sensitivity. Thus, differences exist in the level of endogenous virus expression in cells of different mammalian species.

MeSH Terms
Animals Antigens, Viral/analysis Cats Cell Line Cells, Cultured Chromatography, Ion Exchange Cricetinae Culture Techniques Electrophoresis, Polyacrylamide Gel Epitopes Haplorhini Hominidae Idoxuridine/pharmacology Iodine Radioisotopes Isoelectric Focusing Leukemia Virus, Feline/immunology Leukemia Virus, Murine/immunology Mice RNA Viruses/immunology Radioimmunoassay Rats Rauscher Virus/immunology Retroviridae/immunology Species Specificity
Chemicals
Antigens, Viral Epitopes Iodine Radioisotopes Idoxuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stephenson J R
Aaronson S A
References (28)
28 references, click to expand
  1. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses.
    J Exp Med. 1966 Oct 1;124(4):753-72 PMID: 4288480
  2. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.
    J Bacteriol. 1966 Oct;92(4):1133-40 PMID: 5926742
  3. Spectrophotometric determination of microgram quantities of protein without nucleic acid interference.
    Anal Biochem. 1968 Feb;22(2):195-210 PMID: 4171014
  4. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81 PMID: 5244736
  5. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
    J Cell Physiol. 1968 Oct;72(2):141-8 PMID: 4301006
  6. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
    J Virol. 1969 Nov;4(5):549-53 PMID: 4311790
  7. Shared viral antigen of mammalian leukaemia viruses.
    Nature. 1970 Apr 18;226(5242):265-6 PMID: 4314416
  8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  9. Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):366-76 PMID: 4318783
  10. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses.
    Nat New Biol. 1971 May 26;231(21):107-8 PMID: 4103327
  11. Rescue of the genome of focus forming virus from rat non-productive lines by 5'-bromodeoxyruidine.
    Nat New Biol. 1971 Nov 3;234(44):12-4 PMID: 4330168
  12. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine.
    Science. 1971 Oct 8;174(4005):155-6 PMID: 4330367
  13. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells.
    Science. 1971 Oct 8;174(4005):157-9 PMID: 5119627
  14. The group-specific antigen and other structural proteins of hamster and mouse C-type viruses.
    Virology. 1971 Mar;43(3):665-74 PMID: 4330369
  15. Chemical induction of focus-forming virus from nonproducer cells transformed by murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3069-72 PMID: 4332005
  16. Immunological characterization of primate C-type virus reverse transcriptases.
    Nat New Biol. 1972 Jan 12;235(54):35-40 PMID: 4332802
  17. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses.
    J Virol. 1972 Feb;9(2):359-66 PMID: 4335524
  18. Genetic factors influencing C-type RNA virus induction.
    J Exp Med. 1972 Jul 1;136(1):175-84 PMID: 4113509
  19. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766-70 PMID: 4505653
  20. A rapid direct radioimmunoassay for type C virus group-specific antigen and antibody.
    Virology. 1972 Oct;50(1):294-6 PMID: 4343114
  21. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice.
    J Exp Med. 1972 Nov 1;136(5):1272-85 PMID: 4343244
  22. A genetic locus for inducibility of C-type in BALB-c cells: the effect of a nonlinked regulatory gene on detection of virus after chemical activation.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2798-801 PMID: 4344375
  23. Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.
    J Exp Med. 1973 Mar 1;137(3):622-35 PMID: 4347595
  24. Segregation of loci for C-type virus induction in strains of mice with high and low incidence of leukemia.
    Science. 1973 May 25;180(4088):865-6 PMID: 4122421
  25. Radioimmunoassay for avian C-type virus group-specific antigen: detection in normal and virus-transformed cells.
    J Virol. 1973 Jun;11(6):893-9 PMID: 4351459
  26. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus.
    Virology. 1973 Jul;54(1):199-206 PMID: 4351609
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  28. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.3.564-569.1973
Published
1973-09-00
Pages
564-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356663
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.3.564-569.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2