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PMID: 4128383 Published · ppublish English Comparative Study Journal Article

Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid.

Journal of virology ·Vol. 12 ·No. 6 ·1973-12-00 ·Pages 1560-7

Olsen RH, Thomas DD

Abstract

A new drug resistance plasmid-dependent RNA containing phage resembling coliphage f2 in its particle size and density is described. The phage, PRR1, will only productively infect some R(+) hosts containing the Pseudomonas drug resistance plasmid R1822. The membrane filter-salt elution patterns, RNase sensitivity, inactivation in low ionic strength solutions, and host range serve to distinguish PRR1 from coliphage f2 and two other Pseudomonas RNA phages, 7s and PP7.

MeSH Terms
Bacteriophages/analysis,growth & development,isolation & purification Centrifugation, Density Gradient Chemical Precipitation Coliphages/analysis Drug Resistance, Microbial Enterobacteriaceae Extrachromosomal Inheritance Micropore Filters Microscopy, Electron Phosphotungstic Acid Pseudomonas/drug effects RNA, Viral/analysis Ribonucleases Staining and Labeling Viral Proteins
Chemicals
RNA, Viral Viral Proteins Phosphotungstic Acid Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Olsen R H
Thomas D D
References (13)
13 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1973-12-00
Pages
1560-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356799
Subset
IM
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