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PMID: 4128448 Published · ppublish English Journal Article

Clonal nature of the immune response to phosphorylcholine. I. Specificity, class, and idiotype of phosphorylcholine-binding receptors on lymphoid cells.

The Journal of experimental medicine ·Vol. 139 ·No. 1 ·1974-01-01 ·Pages 58-73

Claflin JL, Lieberman R, Davie JM

Abstract

The relationship between receptor molecules on antigen-binding lymphocytes (ABC) and antibody produced by antibody-secreting cells was studied in inbred strains of mice using the immune response to phosphorylcholine (PC) as a model system. Splenic and lymph node lymphocytes of nonimmune mice possess rare lymphocytes which bind (125)I-labeled PC-bovine serum albumin. The frequency of PC-ABC increases after immunization and is paralleled by a rise in the frequency of PC-specific antibody-producing cells. Both of these responses are thymus independent. The receptors on these ABC display specificity for PC and are exclusively of the IgM class. In one of the strains, BALB/c, the receptors possess the same idiotype and fine degree of specificity for PC and two of its analogues, glycerophosphorylcholine and choline, that are characteristic of a PC-binding myeloma, HOPC 8. Furthermore, the idiotype and class of the receptor in these mice do not change during the course of the immune response. These data provide more direct evidence for the immunelogic relevance of receptor-bearing lymphocytes.

MeSH Terms
Animals Antibody Formation Antibody-Producing Cells Binding Sites, Antibody Cell Membrane/immunology Chlorides Choline Clone Cells Epitopes Immunoglobulins/analysis Iodine Radioisotopes Kinetics Lymphocytes/immunology Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred CBA Microscopy, Fluorescence Organophosphorus Compounds Plasmacytoma/immunology Spleen/cytology Thymus Gland/immunology
Chemicals
Chlorides Epitopes Immunoglobulins Iodine Radioisotopes Organophosphorus Compounds Choline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Claflin J L
Lieberman R
Davie J M
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41 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.139.1.58
Published
1974-01-01
Pages
58-73
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139514
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.139.1.58 PMC Full Text View journal details
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