Antigenic and biochemical characterization of the C-type particle of the stable porcine kidney cell line PK-15.

Journal of virology ·Vol. 12 ·No. 5 ·1973-11-00 ·Pages 1184-6

Woods WA, Papas TS, Hirumi H, Chirigos MA

Abstract

The C-type particles observed by electron microscopy in PK-15 cells were demonstrated to have biochemical and biophysical properties associated with the oncornavirus group: density of 1:16 in a sucrose gradient, 70S RNA, and the RNA-dependent DNA polymerase. The group-specific interspecies antigen, gs-3, was not present. Evidence of a latent infection with a porcine parvovirus was also obtained.

MeSH Terms

Animals Cell Line Centrifugation, Density Gradient DNA-Directed RNA Polymerases Fluorescent Antibody Technique Kidney Microscopy, Electron Oncogenic Viruses/immunology Pancreas/enzymology Parvoviridae/immunology RNA-Directed DNA Polymerase/metabolism Rabbits/immunology Retroviridae/enzymology,immunology,metabolism Strontium Radioisotopes Swine Tritium Uridine/metabolism

Chemicals

Strontium Radioisotopes Tritium RNA-Directed DNA Polymerase DNA-Directed RNA Polymerases Uridine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Woods W A

Papas T S

Hirumi H

Chirigos M A

References (11)

11 references, click to expand

Isolation of noninfectious particles containing Rous sarcoma virus RNA from the medium of Rous sarcoma virus-transformed nonproducer cells.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1655-62 PMID: 4292338
Experimental myeloproliferative and lymphoproliferative diseases of swine.
Bibl Haematol. 1968;30:255-62 PMID: 5244766
Virus-like particles occurring in cultures of stable pig kidney cell lines. Brief report.
Arch Gesamte Virusforsch. 1970;30(4):401-4 PMID: 4195629
An immunofluorescent focus assay for gross leukemia virus.
Proc Soc Exp Biol Med. 1970 Dec;135(3):772-7 PMID: 4922355
C-type virus particles in pig kidney cell lines.
J Gen Virol. 1971 Feb;10(2):195-8 PMID: 4324256
Electron microscopy of Friend murine leukemia virus in the mid-gut of experimentally infected mosquitoes.
J Virol. 1971 Nov;8(5):801-4 PMID: 4108934
A novel DNA polymerase activity found in association with intracisternal A-type particles.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1531-6 PMID: 4504367
RNA tumor viruses. Terminology and ultrastructural aspects of virion morphology and replication.
J Natl Cancer Inst. 1972 Aug;49(2):323-7 PMID: 5076818
Improvements in epoxy resin embedding methods.
J Biophys Biochem Cytol. 1961 Feb;9:409-14 PMID: 13764136
A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.
J Cell Biol. 1965 May;25:407-8 PMID: 14287192
The detection and study of tumor viruses with the electron microscope.
Cancer Res. 1960 Jun;20:712-27 PMID: 13799753

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.5.1184-1186.1973

Published

1973-11-00

Pages

1184-6

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356752

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.5.1184-1186.1973 PMC Full Text View journal details

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Antigenic and biochemical characterization of the C-type particle of the stable porcine kidney cell line PK-15.

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