Primate RNA tumor virus-like DNA synthesized endogenously by RNA-dependent DNA polymerase in virus-like particles from fresh human acute leukemic blood cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 11 ·1973-11-00 ·Pages 3219-24

Gallo RC, Miller NR, Saxinger WC, Gillespie D

Abstract

A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presence of actinomycin D hybridized to RNA isolated from type-C sarcoma viruses of primates or mice; lower annealing values were obtained with RNA isolated from other sarcoma or leukemia viruses. The results confirm and extend previous results from molecular hybridization experiments related to the existence in human leukemia of components of RNA tumor viruses.

MeSH Terms

Adult Avian Leukosis Virus/analysis DNA, Viral/biosynthesis,isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Female Gammaretrovirus/analysis Humans Leukemia Virus, Feline/analysis Leukemia, Myeloid, Acute/microbiology Male Middle Aged Nucleic Acid Hybridization Oncogenic Viruses/analysis RNA Viruses/isolation & purification,metabolism RNA, Viral/isolation & purification,metabolism RNA-Directed DNA Polymerase/metabolism Rauscher Virus/analysis Retroviridae/analysis Tritium

Chemicals

DNA, Viral RNA, Viral Tritium RNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gallo R C

Miller N R

Saxinger W C

Gillespie D

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.11.3219

Published

1973-11-00

Pages

3219-24

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427204

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.11.3219 PMC Full Text View journal details

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Primate RNA tumor virus-like DNA synthesized endogenously by RNA-dependent DNA polymerase in virus-like particles from fresh human acute leukemic blood cells.

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