Abstract
Antisera were raised to a partially purified preparation of human liver hexosaminidase and to highly purified preparations of hexosaminidase isoenzymes A and B. All the antisera precipitated the enzyme in an enzymically active form, which could be located on immunodiffusion and immunoelectrophoretic gels by using a histochemical substrate. The antisera to the purified isoenzymes were shown to react with hexosaminidase from human liver, kidney, brain and spleen, but did not cross-react with human liver beta-glucosidase, beta-galactosidase, alpha-mannosidase, beta-xylosidase, arylsulphatase or acid phosphatase. Hexosaminidases A and B were immunologically identical. The immunological properties of the hexosaminidases from livers of patients with three types of GM(2)-gangliosidoses were closely similar. No evidence could be found for cross-reacting material in enzyme-deficient states.
MeSH Terms
Acetamides
Animals
Chromatography, DEAE-Cellulose
Chromatography, Gel
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Gangliosides
Hexosaminidases/isolation & purification,metabolism
Humans
Immune Sera
Immunodiffusion
Immunoelectrophoresis
Isoelectric Focusing
Isoenzymes/metabolism
Lipid Metabolism, Inborn Errors/enzymology
Liver/enzymology
Microchemistry
Rabbits/immunology
Spectrometry, Fluorescence
Chemicals
Acetamides
Gangliosides
Immune Sera
Isoenzymes
Hexosaminidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carroll M
Robinson D
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