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PMID: 4279906 Published · ppublish English Journal Article

Ribosomal resistance to streptomycin and spectinomycin in Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 120 ·No. 3 ·1974-12-00 ·Pages 1293-9

Maness MJ, Foster GC, Sparling PF

Abstract

A cell-free protein synthesizing system was used to study the mechanism of resistance to streptomycin (Str) and spectinomycin (Spc) in laboratory mutants and clinical isolates of Neisseria gonorrhoeae. The 70S ribosomes from sensitive strains were sensitive to the effects of Str and Spc on synthesis directed by several synthetic polynucleotide messengers, whereas 70S ribosomes from resistant strains were resistant to these same effects. In each case, the alteration was localized to the 30S ribosomal subunit by studying antibiotic sensitivities of hybrid 70S ribosomes formed by combining subunits from sensitive and resistant strains. No evidence was found for streptomycin- or spectinomycin-inactivating enzymes.

MeSH Terms
Amino Acids/metabolism Bacterial Proteins/biosynthesis Carbon Radioisotopes Cell Fractionation Cell-Free System Drug Resistance, Microbial Magnesium/pharmacology Micropore Filters Mutation Neisseria gonorrhoeae/drug effects,ultrastructure Polynucleotides/metabolism RNA, Messenger/metabolism Ribosomes/drug effects,metabolism Spectinomycin/pharmacology Streptomycin/pharmacology
Chemicals
Amino Acids Bacterial Proteins Carbon Radioisotopes Polynucleotides RNA, Messenger Spectinomycin Magnesium Streptomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maness M J
Foster G C
Sparling P F
References (19)
19 references, click to expand
  1. Antibiotic inhibitors of the bacterial ribosome.
    Bacteriol Rev. 1968 Dec;32(4 Pt 2):493-528 PMID: 4179192
  2. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
    J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
  3. Effect of spectinomycin on polypeptide synthesis in extracts of Escherichia coli.
    J Mol Biol. 1967 Oct 14;29(1):203-15 PMID: 4228335
  4. Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli.
    Science. 1968 Jul 4;165(3888):85-6 PMID: 4892072
  5. Mechanisms of antibiotic resistance in bacteria.
    Annu Rev Biochem. 1973;42:471-506 PMID: 4581231
  6. The genetics of bacterial ribosomes.
    Annu Rev Genet. 1972;6:203-34 PMID: 4269096
  7. Selective filtration of antibiotics through collodion membranes.
    Antimicrob Agents Chemother. 1973 Sep;4(3):337-42 PMID: 4758836
  8. DISAPPEARANCE OF OXYTETRACYCLINE ACCUMULATION IN THE CELLS OF MULTIPLE DRUG-RESISTANT ESCHERICHIA COLI.
    Nature. 1963 Oct 26;200:384-5 PMID: 14087909
  9. Ribosomes from Escherichia coli merodiplods heterozygous for resistance to streptomycin and to spectinomycin.
    J Mol Biol. 1968 Nov 14;37(3):407-21 PMID: 4238086
  10. Bacterial ribosome.
    Bacteriol Rev. 1970 Sep;34(3):228-77 PMID: 16350216
  11. Ribosomal proteins. XXVII. Localization of the amino acid exchanges in protein S5 from two Escherichia coli mutants resistant to spectinomycin.
    Mol Gen Genet. 1972;114(2):106-11 PMID: 4553095
  12. STREPTOMYCIN, SUPPRESSION, AND THE CODE.
    Proc Natl Acad Sci U S A. 1964 May;51:883-90 PMID: 14173007
  13. SOME PHYSICAL PROPERTIES OF BACTERIOPHAGE R17 AND ITS RIBONUCLEIC ACID.
    J Mol Biol. 1964 Apr;8:496-507 PMID: 14153522
  14. Ribosomal proteins. 33. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin.
    J Mol Biol. 1972 Jul 28;68(3):547-50 PMID: 4560854
  15. Inhibition of protein synthesis by spectinomycin.
    Science. 1965 Sep 3;149(3688):1096-8 PMID: 5318092
  16. Pairing of inosine with adenosine in codon position two in the translation of polyinosinic acid.
    J Mol Biol. 1973 May 15;76(2):223-32 PMID: 4579546
  17. Mechanism of resistance to antibiotic synergism in enterococci.
    J Bacteriol. 1971 Mar;105(3):873-9 PMID: 4994038
  18. STUDIES ON THE RIBOSOMES OF STREPTOMYCIN-SENSITIVE AND RESISTANT STRAINS OF ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1964 Apr;51:659-64 PMID: 14166773
  19. Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7 PMID: 4943794
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.3.1293-1299.1974
Published
1974-12-00
Pages
1293-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245914
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.3.1293-1299.1974 PMC Full Text View journal details
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