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PMID: 4309122 Published · ppublish English Journal Article

Phosphatidate biosynthesis in mitochondrial subfractions of rat liver.

The Biochemical journal ·Vol. 113 ·No. 2 ·1969-06-00 ·Pages 429-40

Shephard EH, Hübscher G

Abstract

1. After conventional fractionation of rat liver homogenates in 0.88m-sucrose the mitochondrial fraction was subjected to short-term water lysis followed by separation of the resulting membrane preparations. 2. Phosphatidate formation was measured in all subcellular fractions and subfractions and was compared with the distribution of succinate dehydrogenase, monoamine oxidase, rotenone-insensitive NADH cytochrome c reductase, arylsulphatase, urate oxidase, arylesterase and glucose 6-phosphatase. 3. The results obtained indicated that mitochondria were capable of synthesizing phosphatidate, though this activity was only about one-third of the total homogenate activity. 4. Mitochondrial phosphatidate formation was located predominantly in the outer mitochondrial membrane. Although this membrane preparation was found to be significantly contaminated by the microsomal fraction, this contamination was estimated to account for not more than about 20% of the total phosphatidate formation observed in preparations of outer mitochondrial membrane.

MeSH Terms
Animals Centrifugation, Density Gradient Glucose-6-Phosphatase/analysis Glycerides/biosynthesis In Vitro Techniques Membranes/enzymology Mitochondria, Liver/enzymology,metabolism Monoamine Oxidase/analysis Oxidoreductases/analysis Phospholipids/biosynthesis Rats Succinate Dehydrogenase/analysis Sulfatases/analysis Urate Oxidase/analysis
Chemicals
Glycerides Phospholipids Oxidoreductases Succinate Dehydrogenase Monoamine Oxidase Urate Oxidase Glucose-6-Phosphatase Sulfatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shephard E H
Hübscher G
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45 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1130429
Published
1969-06-00
Pages
429-40
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1184651
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1130429 PMC Full Text View journal details
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