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PMID: 4322872 Published · ppublish English Journal Article

Virus-specific deoxyribonucleic acid in simian virus 40-exposed hamster cells: correlation with S and T antigens.

Journal of virology ·Vol. 6 ·No. 2 ·1970-08-00 ·Pages 199-207

Levine AS, Oxman MN, Henry PH, Levin MJ, Diamandopoulos GT, Enders JF

Abstract

Several homologous hamster embryonic cell lines, transformed in association with simian virus (SV) 40 infection, were examined for the presence of deoxyribonucleic acid (DNA) complementary to SV40 ribonucleic acid (RNA) made in vitro. The methods employed permitted the detection of 10(-5) mug of viral DNA in 100 mug of cellular DNA, corresponding to one-fifth of an SV40 DNA molecule per cell. Those lines which contained both the SV40 surface (S) and tumor (T) antigens also contained DNA complementary to SV40 RNA synthesized in vitro. In contrast, neither of two lines which contained S, but not T, antigen contained detectable DNA complementary to SV40 RNA. These findings suggest that the production of S antigen does not depend upon the persistence of SV40 DNA in transformed cells.

MeSH Terms
Animals Antigens/analysis Carbon Isotopes Cell Line Cell Transformation, Neoplastic Centrifugation, Density Gradient Cricetinae Culture Techniques DNA/analysis DNA, Viral/analysis,isolation & purification Fluorescent Antibody Technique Genetics, Microbial Haplorhini Hybridization, Genetic Immune Sera Kidney Mice RNA, Viral/biosynthesis Simian virus 40/analysis,growth & development,immunology,pathogenicity Sucrose Tritium
Chemicals
Antigens Carbon Isotopes DNA, Viral Immune Sera RNA, Viral Tritium Sucrose DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Levine A S
Oxman M N
Henry P H
Levin M J
Diamandopoulos G T
Enders J F
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26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.6.2.199-207.1970
Published
1970-08-00
Pages
199-207
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376109
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.6.2.199-207.1970 PMC Full Text View journal details
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