Role of the receptor in the mechanism of action of adenosine 3':5'-cyclic monophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 4 ·1971-04-00 ·Pages 786-90

Gill GN, Garren LD

Abstract

Highly purified cAMP-dependent protein phosphokinase from adrenal-cortical tissue contains cAMP-receptor activity. In activating the kinase, cAMP binds to the receptor and causes it to dissociate from its complex with the kinase. The kinase, freed of receptor, is fully activated and no longer stimulable by cAMP. Kinase can be similarly activated by differentially denaturing the receptor with heat. Addition of receptor suppresses kinase activity; this suppression can be overcome by cAMP. After dissociation of receptor, two molecular forms of the activated kinase exist. The cAMP receptor thus functions as a repressor of the protein kinase; binding of cAMP to receptor causes it to dissociate from the kinase, which is then fully activated.

MeSH Terms

Adenine Nucleotides Adrenal Glands/analysis Animals Cattle Chemical Phenomena Chemistry Chromatography, DEAE-Cellulose Cyclic AMP/pharmacology Electrophoresis Hot Temperature In Vitro Techniques Phosphorus Isotopes Phosphotransferases/isolation & purification,pharmacology Protein Binding Proteins/isolation & purification,pharmacology Tritium Ultracentrifugation

Chemicals

Adenine Nucleotides Phosphorus Isotopes Proteins Tritium Cyclic AMP Phosphotransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gill G N

Garren L D

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22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.4.786

Published

1971-04-00

Pages

786-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389043

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.4.786 PMC Full Text View journal details

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Role of the receptor in the mechanism of action of adenosine 3':5'-cyclic monophosphate.

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