Production and evaluation of a purified adenovirus group-specific (hexon) antigen for use in the diagnostic complement fixation test.

Applied microbiology ·Vol. 21 ·No. 4 ·1971-04-00 ·Pages 718-22

Dowdle WR, Lambriex M, Hierholzer JC

Abstract

A simple procedure for the production of large volumes of purified adenovirus group-specific complement-fixing (CF) (hexon) antigen by selective adsorption to and elution from CaHPO(4) is described. Results of immunodiffusion tests, electrophoresis, electron microscopy, and tests for hemagglutination and infectivity indicate that the purified antigen consisted of a single virus component (hexon). The purified product contained little host materials. Unlike the crude virus harvest usually employed for serodiagnostic CF tests, the purified antigen demonstrated no anticomplementary activity and did not develop such activity during storage. The purified antigen was equal to or slightly more sensitive than crude virus harvests for serodiagnosis of adenovirus infections.

MeSH Terms

Adenoviridae/growth & development,immunology,pathogenicity Adenoviridae Infections/diagnosis Adsorption Antigens/isolation & purification Buffers Calcium Phosphates Carcinoma Cell Line Chromatography Complement Fixation Tests Culture Media Diagnosis, Differential Dialysis Electrophoresis Hemagglutination Tests Humans Immune Sera Immunoelectrophoresis Laryngeal Neoplasms Methods Microscopy, Electron Sodium Sulfates

Chemicals

Antigens Buffers Calcium Phosphates Culture Media Immune Sera Sulfates Sodium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dowdle W R

Lambriex M

Hierholzer J C

References (12)

12 references, click to expand

The purification and concentration of viruses and virus soluble antigens on calcium phosphate.
J Gen Microbiol. 1958 Dec;19(3):451-61 PMID: 13611187
Immunological basis of the adenovirus 8-9 cross-reaction.
J Virol. 1970 Dec;6(6):782-7 PMID: 4992997
Neutralizing, hemagglutination-inhibiting and group complement-fixing antibody responses in human adenovirus infections.
J Immunol. 1966 Jul;97(1):64-74 PMID: 4288350
Identification and classification of adenoviruses.
Virology. 1962 Oct;18:312-9 PMID: 13948036
The sensitivity of the complement fixation test for the detection of adenovirus infections in infants and children with lower respiratory disease.
Am J Epidemiol. 1967 Sep;86(2):362-71 PMID: 4293745
Chromatography of adenoviruses on calcium phosphate columns.
Acta Virol. 1962 Jul;6:302-8 PMID: 14039293
Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation.
Appl Microbiol. 1969 Nov;18(5):824-33 PMID: 4984203
Hemagglutination-inhibition antibody responses in human adenovirus infections.
Proc Soc Exp Biol Med. 1961 Nov;108:474-9 PMID: 14493909
The structural and functional diversity of Adenovirus capsid components.
J Gen Virol. 1969 Sep;5(2):221-36 PMID: 4899805
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Architecture of the Adenovirus Capsid.
J Bacteriol. 1965 Jul;90(1):254-61 PMID: 16562026
Recovery and identification of adenovirus in infections of infants and children.
Bacteriol Rev. 1965 Dec;29(4):487-95 PMID: 4285159

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.21.4.718-722.1971

Published

1971-04-00

Pages

718-22

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377262

Subset

External Links

PubMed source DOI: 10.1128/am.21.4.718-722.1971 PMC Full Text

Analysis Services

Production and evaluation of a purified adenovirus group-specific (hexon) antigen for use in the diagnostic complement fixation test.

Contact

WeChat Official Account