Dose responsiveness and variation among inbred strains of mice in production of interferon after treatment with poly (I)-poly (C) (poly-D-lysine) complexes.

Applied microbiology ·Vol. 22 ·No. 3 ·1971-09-00 ·Pages 380-6

Rice JM, Turner W, Chirigos MA, Spahn G

Abstract

Poly-d-lysine forms a stochiometric complex with poly(I) . poly(C) which has a higher T(m) (83 C in 0.15 m NaCl) than the uncomplexed double-stranded polyribo-nucleotide (63 C). The complex was superior to poly(I) . poly(C) alone as an interferon inducer in vivo. Significant serum interferon titers were attained in Swiss mice during a 24-hr period after intraperitoneal injection of 10 to 300 mug of poly(I) . poly(C) [1.0 poly-d-lysine] complex, at concentrations of 100 to 1,000 mug/ml. The serum interferon responses (average and maximum titers) of a series of inbred strains of mice to a single intraperitoneal injection of 100 mug of complex decreased in the order: Swiss > DBA/2 > C3H > BALB/c > CF-1 > AKR, C57Bl/6, NZB > SJL > NZW and varied by a factor of approximately 100 from the most to the least responsive.

MeSH Terms

Animals Biological Assay Chemical Phenomena Chemical Precipitation Chemistry Genetics Injections, Intraperitoneal Interferons/analysis,biosynthesis,blood L Cells Lysine/administration & dosage Mice Mice, Inbred Strains/drug effects,metabolism Molecular Weight Poly I-C/administration & dosage Polynucleotides/administration & dosage Species Specificity Spectrum Analysis Time Factors Ultraviolet Rays Vesicular stomatitis Indiana virus

Chemicals

Polynucleotides Interferons Lysine Poly I-C

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rice J M

Turner W

Chirigos M A

Spahn G

References (21)

21 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.22.3.380-386.1971

Published

1971-09-00

Pages

380-6

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC376318

Subset

External Links

PubMed source DOI: 10.1128/am.22.3.380-386.1971 PMC Full Text

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Dose responsiveness and variation among inbred strains of mice in production of interferon after treatment with poly (I)-poly (C) (poly-D-lysine) complexes.

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