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PMID: 4338641 Published · ppublish English Journal Article

Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells.

Journal of virology ·Vol. 9 ·No. 6 ·1972-06-00 ·Pages 956-68

Robb JA, Martin RG

Abstract

A temperature-sensitive mutant of simian virus 40 (SV40), ts(*)101, has been characterized during productive infection in monkey kidney cells. The mutant virion can adsorb to and penetrate the cell normally at the restrictive temperature, but cannot induce the synthesis of cellular deoxyribonucleic acid (DNA) nor initiate the synthesis of SV40-specific tumor, virion, or U antigens or viral DNA. First-cycle infection with purified ts(*)101 DNA is normal at the restrictive temperature, but the resulting progeny virions are still temperature-sensitive. The mutant neither complements nor inhibits other temperature-sensitive SV40 mutants or wild-type virions. The affected protein in the ts(*)101 mutant may be a regulatory structural protein, possibly a core protein, that is interacting with the viral DNA.

MeSH Terms
Adsorption Animals Antigens, Viral/metabolism Carbon Isotopes Cell Line/microbiology Cycloheximide/pharmacology DNA/biosynthesis DNA, Viral/biosynthesis,pharmacology Genetic Complementation Test Genetics, Microbial Haplorhini Kidney Mutation Simian virus 40/immunology,metabolism Temperature Thymidine/metabolism Time Factors Tritium Viral Proteins/biosynthesis Virus Replication
Chemicals
Antigens, Viral Carbon Isotopes DNA, Viral Viral Proteins Tritium DNA Cycloheximide Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robb J A
Martin R G
References (39)
39 references, click to expand
  1. Site of host restriction of simian virus 40 mutants in an established African green monkey kidney cell line.
    J Virol. 1969 Oct;4(4):408-15 PMID: 4309877
  2. Bacteriophage P2: replication of the chromosome requires a protein which acts only on the genome that coded for it.
    Virology. 1970 Oct;42(2):522-33 PMID: 5489227
  3. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  4. More mutant tyrosine transfer ribonucleic acids.
    J Mol Biol. 1970 Nov 28;54(1):1-14 PMID: 4923867
  5. Susceptibility of human cell strains to transformation by simian virus 40 and simian virus 40 deoxyribonucleic acid.
    J Virol. 1970 Oct;6(4):470-5 PMID: 5497893
  6. Inactivating and mutagenic effects of nitrosoguanidine on simian virus 40.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):745-52 PMID: 4316681
  7. Positive control of transcription by a bacteriophage sigma factor.
    Nature. 1970 Mar 14;225(5237):1009-12 PMID: 5416465
  8. Simian virus 40 deoxyribonucleic acid transcription in vitro: binding and transcription patterns with a mammalian ribonucleic acid polymerase.
    J Virol. 1970 Nov;6(5):667-76 PMID: 4320700
  9. Capsid proteins of Simian virus 40.
    Biochem Biophys Res Commun. 1970 Jul 13;40(1):97-102 PMID: 4318589
  10. On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407-11 PMID: 4944622
  11. The basis for variation in susceptibility to poliovirus in HeLa cells.
    Virology. 1960 Aug;11:665-75 PMID: 13814144
  12. Stages in phage R17 infection. VI. Injection of A protein and RNA into the host cell.
    Virology. 1972 Mar;47(3):628-37 PMID: 4551992
  13. Complementation and transformation by temperature-sensitive mutants of polyoma virus.
    Virology. 1969 May;38(1):120-5 PMID: 4306521
  14. Fate of maturation protein during infection by coliphage MS2.
    Nat New Biol. 1971 Sep 15;234(50):209-11 PMID: 4942983
  15. Strand-specific supercoiled DNA-protein relaxation complexes: comparison of the complexes of bacterial plasmids ColE1 and ColE2.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):210-4 PMID: 4924969
  16. Rapid detection of mycoplasma-infected cell cultures.
    Exp Cell Res. 1971 Mar;65(1):256-7 PMID: 4994336
  17. Early events in the infection of permissive cells with simian virus 40: adsorption, penetration, and uncoating.
    J Virol. 1970 Jul;6(1):78-86 PMID: 4097232
  18. Temperature-sensitive mutants of polyoma virus.
    Virology. 1969 May;38(1):126-33 PMID: 4306522
  19. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  20. Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus.
    Virology. 1970 Aug;41(4):751-60 PMID: 4319784
  21. Temperature-sensitive simian virus 40 mutant defective in a late function.
    J Virol. 1970 Sep;6(3):286-94 PMID: 4320387
  22. Morphological aspects of the uptake of simian virus 40 by permissive cells.
    J Virol. 1970 Jul;6(1):87-93 PMID: 4097233
  23. INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS.
    Proc Natl Acad Sci U S A. 1964 Jul;52:53-9 PMID: 14192657
  24. Structural polypeptides of simian virus 40.
    J Virol. 1971 May;7(5):635-41 PMID: 4326753
  25. Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity.
    J Bacteriol. 1966 Oct;92(4):990-4 PMID: 4288804
  26. Proposal for a uniform nomenclature for simian virus 40 mutants.
    J Virol. 1972 Mar;9(3):562-3 PMID: 4335666
  27. Circular DNA.
    Annu Rev Biochem. 1971;40:899-942 PMID: 4942224
  28. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.
    J Exp Med. 1958 Dec 1;108(6):945-56 PMID: 13598821
  29. Temperature-sensitive mutants of simian virus 40: infection of permissive cells.
    J Virol. 1971 Oct;8(4):516-24 PMID: 4331655
  30. Characterization of a temperature-sensitive mutant of polyoma virus.
    Virology. 1970 Mar;40(3):605-17 PMID: 4314508
  31. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells.
    J Virol. 1972 Jan;9(1):41-51 PMID: 4333544
  32. Genetic analysis of simian virus 40. IV. Inhibited transformation of Balb-3T3 cells by a temperature-sensitive early mutant.
    J Virol. 1972 Jun;9(6):969-72 PMID: 4338642
  33. Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of primary infected Chinese hamster cells.
    J Virol. 1971 Nov;8(5):708-15 PMID: 4332141
  34. Isolation of a polyoma-nucleoprotein complex from infected mouse-cell cultures.
    Proc Natl Acad Sci U S A. 1971 May;68(5):1032-6 PMID: 4324998
  35. Induction of DNA synthesis by SV40.
    Proc Natl Acad Sci U S A. 1966 Aug;56(2):736-40 PMID: 4291013
  36. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.
    J Natl Cancer Inst. 1968 Aug;41(2):351-7 PMID: 4299537
  37. The induction of cellular DNA synthesis by simian virus 40 in contact-inhibited and in x-irradiated cells.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):918-25 PMID: 16578647
  38. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128-35 PMID: 4311250
  39. Regulation of lac mRNA synthesis in a soluble cell-free system.
    Nat New Biol. 1971 Mar 10;230(10):37-40 PMID: 4324112
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.9.6.956-968.1972
Published
1972-06-00
Pages
956-68
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356401
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.9.6.956-968.1972 PMC Full Text View journal details
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