Home LiteratureArticle Details
PMID: 4342002 Published · ppublish English Journal Article

The effect of adenosine on the release of the transmitter from the phrenic nerve of the rat.

The Journal of physiology ·Vol. 224 ·No. 3 ·1972-08-00 ·Pages 629-45

Ginsborg BL, Hirst GD

Abstract

1. The action of adenosine on neuromuscular transmission has been studied on the rat phrenic nerve-diaphragm preparation.2. Adenosine (0.025-10 mM) reversibly reduced the quantum content of end-plate potentials and the frequency of miniature end-plate potentials to about half the control values in preparations in which transmission was blocked with high Mg(2+) and/or low Ca(2+) concentrations. Where transmission was blocked with tubocurarine, the amplitude of end-plate potentials was reduced to about half the control values by adenosine.3. In solutions with very low Ca(2+) concentrations (no added Ca(2+) and 1 mM-EGTA) adenosine had a smaller effect on the frequency of miniature end-plate potentials.4. The effect of adenosine (0.025 and 0.05 mM) was abolished by theophylline (1.8 mM).5. The results are discussed in relation to the increase in cyclic AMP in brain slices caused by adenosine and the abolition of this effect by theophylline (Sattin & Rall, 1970).

MeSH Terms
Adenosine/antagonists & inhibitors,pharmacology Animals Calcium/pharmacology Cyclic AMP/metabolism,physiology Diaphragm/innervation,physiology Electric Stimulation Electrophysiology In Vitro Techniques Magnesium/pharmacology Male Neuromuscular Junction/drug effects,physiology Phrenic Nerve/physiology Rats Synaptic Transmission/drug effects Theophylline/pharmacology Tubocurarine/pharmacology
Chemicals
Theophylline Cyclic AMP Magnesium Adenosine Calcium Tubocurarine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ginsborg B L
Hirst G D
References (23)
23 references, click to expand
  1. Post-tetanic increase in frequency of miniature end-plate potentials in calcium-free solutions.
    J Physiol. 1967 Sep;192(2):54P-55P PMID: 4292911
  2. Co-operative action a calcium ions in transmitter release at the neuromuscular junction.
    J Physiol. 1967 Nov;193(2):419-32 PMID: 6065887
  3. On the mechanism by which calcium and magnesium affect the spontaneous release of transmitter from mammalian motor nerve terminals.
    J Physiol. 1968 Feb;194(2):355-80 PMID: 4295698
  4. The role of calcium in neuromuscular facilitation.
    J Physiol. 1968 Mar;195(2):481-92 PMID: 4296699
  5. Cyclic adenosine monophosphate, CA++, and membranes.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1364-70 PMID: 4297336
  6. On the mechanism by which calcium and magnesium affect the release of transmitter by nerve impulses.
    J Physiol. 1968 May;196(1):75-86 PMID: 4297537
  7. An examination of the effects of osmotic pressure changes upon transmitter release from mammalian motor nerve terminals.
    J Physiol. 1968 Aug;197(3):639-57 PMID: 4299014
  8. A study of the mechanism of quantal transmitter release at a chemical synapse.
    J Physiol. 1968 Nov;199(1):11-35 PMID: 4300871
  9. Evidence for a role of cyclic AMP in neuromuscular transmission.
    Proc Natl Acad Sci U S A. 1969 Sep;64(1):134-41 PMID: 4312746
  10. The effect of Ca2+ on the adenyl cyclase of calf brain.
    Biochim Biophys Acta. 1970 Feb 24;201(2):250-60 PMID: 4313941
  11. Effects of calcium on the contraction of the hypodynamic frog heart.
    J Physiol. 1970 Dec;211(2):389-421 PMID: 5501007
  12. Adenosine 3',5'-monophosphate in nervous tissue: increase associated with synaptic transmission.
    Science. 1971 Mar 19;171(3976):1156-8 PMID: 4322706
  13. Tetanic and post-tetanic rise in frequency of miniature end-plate potentials in low-calcium solutions.
    J Physiol. 1971 Jan;212(1):245-57 PMID: 5545181
  14. Estimates of probability of transmitter release at the mammalian neuromuscular junction.
    J Physiol. 1970 Nov;210(4):933-45 PMID: 4395959
  15. Effects of iontophoretically applied drugs on spinal interneurons of the lamprey.
    J Physiol. 1970 May;207(3):653-65 PMID: 5503275
  16. Effects of adrenaline on nerve terminals in the superior cervical ganglion of the rabbit.
    Br J Pharmacol. 1971 Feb;41(2):331-8 PMID: 4324594
  17. Calcium: is it required for transmitter secretion?
    Science. 1971 Jun 4;172(3987):1034-6 PMID: 4396796
  18. Cyclic AMP, transmitter release and the effect of adenosine on neuromuscular transmission.
    Nat New Biol. 1971 Jul 14;232(28):63-4 PMID: 4327745
  19. Effects of calcium and magnesium on the frequency of miniature end-plate potentials during prolonged tetanization.
    J Physiol. 1971 Dec;219(1):17-38 PMID: 5316661
  20. The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices.
    Mol Pharmacol. 1970 Jan;6(1):13-23 PMID: 4354003
  21. Spontaneous subthreshold activity at motor nerve endings.
    J Physiol. 1952 May;117(1):109-28 PMID: 14946732
  22. The nature of the antagonism between calcium and magnesium ions at the neuromuscular junction.
    J Physiol. 1957 Oct 30;138(3):434-44 PMID: 13481883
  23. A further study of the statistical composition on the end-plate potential.
    J Physiol. 1955 Oct 28;130(1):114-22 PMID: 13278890
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1972.sp009916
Published
1972-08-00
Pages
629-45
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1331513
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1972.sp009916 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2