Biochemically differentiated neoplastic clone of Schwann cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 2885-9

Pfeiffer SE, Wechsler W

Abstract

Four clonal lines have been established in tissue culture from a transplantable tumor of cervical nerve-root induced transplacentally with ethylnitrosourea in an inbred BD-IX rat strain. One clone (RN-2) has several biochemical properties of the nervous system that led to its classification as a Schwann cell line, namely (a) the synthesis of the nervous system specific protein S-100, (b) a high specific activity of 2',3'-cyclic nucleotide-3'-phosphohydrolase, an enzyme present in high specific activity only in glial cells, and (c) the presence of a basic protein related by immunological crossreaction, molecular size, and amino-acid composition to the encephalitogenic protein from beef brain myelin. Clone RN-2 has a high plating efficiency, a doubling time of about 20 hr, and a mean of 43 chromosomes of normal morphology. Clone RN-2 has also been inoculated subcutaneously into syngeneic rats and grown as a clonal tumor with good growth properties.

MeSH Terms

Amino Acids/analysis Animals Cell Differentiation Cell Transformation, Neoplastic Chromosomes/analysis Clone Cells/metabolism Complement Fixation Tests Cross Reactions Culture Techniques Cyclic AMP Electrophoresis, Polyacrylamide Gel Female Myelin Sheath Neoplasm Transplantation Nerve Tissue Proteins/analysis Neurilemmoma/chemically induced Nitrosourea Compounds Phosphoric Monoester Hydrolases/metabolism Pregnancy Rats Rats, Inbred Strains Schwann Cells/analysis,enzymology,metabolism Uterine Cervical Neoplasms/chemically induced

Chemicals

Amino Acids Nerve Tissue Proteins Nitrosourea Compounds Cyclic AMP Phosphoric Monoester Hydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pfeiffer S E

Wechsler W

References (25)

25 references, click to expand

A soluble protein characteristic of the nervous system.
Biochem Biophys Res Commun. 1965 Jun 9;19(6):739-44 PMID: 4953930
Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype.
Cancer Res. 1966 Mar;26(3):529-35 PMID: 5930699
Establishment of four functional, clonal strains of animal cells in culture.
Science. 1966 Dec 2;154(3753):1186-9 PMID: 4288399
Differentiated rat glial cell strain in tissue culture.
Science. 1968 Jul 26;161(3839):370-1 PMID: 4873531
[S-100 protein and human cerebral tumors].
Rev Neurol (Paris). 1968 May;118(5):368-72 PMID: 4301327
Studies on 2',3'-cyclic nucleotide-3'-phosphohydrolase from brain.
Can J Biochem. 1969 Oct;47(10):961-6 PMID: 4310670
Establishment of functional clonal lines of neurons from mouse neuroblastoma.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):311-5 PMID: 5263016
Allergic encephalomyelitis: preparation of the encephalitogenic basic protein from bovine brain.
Arch Biochem Biophys. 1970 Jun;138(2):392-6 PMID: 5433575
Amino acid sequence of rabbit liver microsomal cytochrome b5.
J Biol Chem. 1970 Oct 10;245(19):4863-74 PMID: 5506260
Amino acid sequence of the basic protein of the myelin membrane.
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1425-31 PMID: 5274468
Transplacental production with ethylnitrosourea of neoplasms of the nervous system in Sprague-Dawley rats.
Am J Pathol. 1971 Apr;63(1):37-56 PMID: 4323476
2',3'-cyclic nucleotide 3'-phosphohydrolase in purified myelin from brain of Jimpy and normal young mice.
Life Sci II. 1971 Apr 22;10(8):421-9 PMID: 4326122
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
Genotypes and phenotypes of ten inbred strains of BD-rats.
Arzneimittelforschung. 1971 Aug;21(8):1274-8 PMID: 5110034
Ethylnitrosourea-induced transplantable tumors of the peripheral nervous system in inbred rats.
Naturwissenschaften. 1971 Nov;58(11):577-8 PMID: 5139299
2',3'-cyclic nucleotide 3'-phosphohydrolase in human erythrocyte membranes.
Biochim Biophys Acta. 1972 Feb 11;255(2):640-6 PMID: 4333432
Biochemically differentiated clonal human glial cells in tissue culture.
J Neurobiol. 1970;1(4):411-7 PMID: 5535723
Isolation and characterization of a protein from sciatic nerve myelin responsible for experimental allergic neuritis.
Nat New Biol. 1972 Feb 16;235(59):210-2 PMID: 4622214
The presence of 2',3'-cyclic AMP 3'-phosphohydrolase in glial cells in tissue culture.
J Neurochem. 1972 Mar;19(3):881-3 PMID: 4337920
S-100 protein in human acoustic neurinomas.
Brain Res. 1972 Jun 8;41(1):187-93 PMID: 5036036
Myelin basic proteins of mammalian and submammalian vertebrates: encephalitogenic activities in guinea pigs and rats.
J Immunol. 1972 Aug;109(2):262-70 PMID: 4114825
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.
J Exp Med. 1958 Dec 1;108(6):945-56 PMID: 13598821
Deoxyribonucleic acid synthesis in individual L-strain mouse cells. II. Effects of thymidine starvation.
Biochim Biophys Acta. 1963 Jun 25;72:277-89 PMID: 13990346
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.
Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 PMID: 14294058

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.10.2885

Published

1972-10-00

Pages

2885-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389667

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.10.2885 PMC Full Text View journal details

Analysis Services

Biochemically differentiated neoplastic clone of Schwann cells.

Contact

WeChat Official Account