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PMID: 4344960 Published · ppublish English Comparative Study Journal Article

Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.

Applied microbiology ·Vol. 24 ·No. 5 ·1972-11-00 ·Pages 801-4

Duncan CL, Somers EB

Abstract

Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 mug of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.

MeSH Terms
Agar Animals Bacteriological Techniques Chromatography, Ion Exchange Clostridium perfringens/analysis Electrophoresis Enterotoxins/analysis,isolation & purification Evaluation Studies as Topic Guinea Pigs Immunodiffusion Serotyping Skin Tests
Chemicals
Enterotoxins Agar
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Duncan C L
Somers E B
References (8)
8 references, click to expand
  1. Rapid quantitative serological assay of staphylococcal enterotoxin B.
    Appl Microbiol. 1966 Mar;14(2):284-91 PMID: 4959985
  2. Studies of electroimmunodiffusion: immunochemical quantitation of proteins in dilute solutions.
    Immunochemistry. 1969 Jul;6(4):513-26 PMID: 4183311
  3. Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens.
    J Bacteriol. 1969 Oct;100(1):86-94 PMID: 4310083
  4. Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A.
    Can J Microbiol. 1970 Aug;16(8):651-4 PMID: 4320760
  5. The role of enterotoxin in Clostridium perfringens type A enteritis.
    Can J Microbiol. 1971 Jul;17(7):987-91 PMID: 4328690
  6. Purification and characteristics of the enterotoxin of Clostridium perfringens type A.
    Can J Microbiol. 1971 Nov;17(11):1425-33 PMID: 4333491
  7. Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion.
    Infect Immun. 1971 Aug;4(2):126-9 PMID: 5005291
  8. Biological characteristics of Clostridium perfringens type A enterotoxin.
    Infect Immun. 1971 Aug;4(2):89-96 PMID: 4343403
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.24.5.801-804.1972
Published
1972-11-00
Pages
801-4
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC380666
Subset
IM
External Links
PubMed source DOI: 10.1128/am.24.5.801-804.1972 PMC Full Text
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