Induction of cytolysis of cultured lymphoma cells by adenosine 3':5'-cyclic monophosphate and the isolation of resistant variants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 76-9

Daniel V, Litwack G, Tomkins GM

Abstract

Cultured mouse lymphosarcoma cells are killed on exposure to 0.1 mM N(6),O(2')-dibutyryl-adenosine 3':5'-cyclic monophosphate. A population of cells resistant to the killing effect of dibutyryl cyclic AMP at concentrations as high as 1 mM was selected. The growth characteristics of the resistant cells were similar to those of the sensitive parental line. However, the resistant cells contain less cytoplasmic cyclic AMP-binding proteins and decreased cyclic AMP-stimulated protein kinase activity. It is proposed that transition from sensitivity to resistance to dibutyryl cyclic AMP in lymphoma cells is connected with a modification of the cyclic AMP-binding protein, which appears to be the regulatory subunit of the cyclic AMP-activated protein kinase.

MeSH Terms

Animals Cell Division/drug effects Cell Line Cell Survival/drug effects Cell-Free System Cells, Cultured Chromatography, Ion Exchange Cyclic AMP/metabolism,pharmacology Enzyme Activation/drug effects Hydrogen-Ion Concentration Lymphoma/enzymology Mice Neoplasm Proteins/isolation & purification,metabolism Phosphotransferases/isolation & purification,metabolism Protein Binding Receptors, Drug Tritium

Chemicals

Neoplasm Proteins Receptors, Drug Tritium Cyclic AMP Phosphotransferases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Daniel V

Litwack G

Tomkins G M

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.76

Published

1973-01-00

Pages

76-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433187

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.76 PMC Full Text View journal details

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Induction of cytolysis of cultured lymphoma cells by adenosine 3':5'-cyclic monophosphate and the isolation of resistant variants.

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