The acid and enzymic hydrolysis of O-acetylated sialic acid residues from rabbit Tamm-Horsfall glycoprotein.

The Biochemical journal ·Vol. 129 ·No. 3 ·1972-09-00 ·Pages 683-93

Neuberger A, Ratcliffe WA

Abstract

Rabbit Tamm-Horsfall glycoprotein and bovine submaxillary glycoprotein were both found to contain sialic acid residues which are released at a slow rate by the standard conditions of acid hydrolysis. These residues are also resistant to neuraminidases from Vibrio cholerae and Clostridium perfringens. This behaviour was attributed to the presence of O-acetylated sialic acid, since the removal of O-acetyl groups by mild alkaline treatment normalized the subsequent release of sialic acid from rabbit Tamm-Horsfall glycoprotein by acid and by enzymic hydrolysis. Determination of the O-acetyl residues in rabbit Tamm-Horsfall glycoprotein indicated that on average two hydroxyl groups of sialic acid are O-acetylated, and these were located on the polyhydroxy side-chain of sialic acid or on C-4 and C-8. These findings confirm the assumption that certain O-acetylated forms of sialic acid are not substrates for bacterial neuraminidases. Several explanations have been suggested to explain the effect of O-acetylation of the side-chain on the rate of acidcatalysed hydrolysis of sialic acid residues.

MeSH Terms

Acetylation Animals Cattle Chromatography, Paper Clostridium perfringens/enzymology Esters/analysis Formaldehyde Glycoproteins/analysis,urine Hydrolysis Neuraminic Acids/analysis Neuraminidase Periodic Acid Rabbits Sodium Hydroxide Submandibular Gland/analysis Sulfuric Acids Vibrio/enzymology

Chemicals

Esters Glycoproteins Neuraminic Acids Sulfuric Acids Periodic Acid Formaldehyde Sodium Hydroxide Neuraminidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Neuberger A

Ratcliffe W A

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25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1290683

Published

1972-09-00

Pages

683-93

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1174170

Subset

External Links

PubMed source DOI: 10.1042/bj1290683 PMC Full Text View journal details

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The acid and enzymic hydrolysis of O-acetylated sialic acid residues from rabbit Tamm-Horsfall glycoprotein.

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