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PMID: 4351461 Published · ppublish English Journal Article

Suppression of amber mutations of bacteriophage T4 gene 43 (DNA polymerase) by translational ambiguity.

Journal of virology ·Vol. 11 ·No. 6 ·1973-06-00 ·Pages 933-45

Karam JD, O'Donnell PV

Abstract

The growth properties of twelve different amber (am) mutants of bacteriophage T4 gene 43 (DNA polymerase) were examined by using nonpermissive (su(-)) as well as permissive (su(+)) Escherichia coli hosts. It was found that most of these mutants were measurably suppressed in su(-) hosts by translational ambiguity (misreading of codons during protein synthesis). The ability of these mutants to grow in response to this form of weak suppression probably means that the T4 gene 43 DNA polymerase can be effective in supporting productive DNA replication when it is supplied in small amounts. By similar criteria, studies with other phage mutants suggested that the products of T4 genes 62 (uncharacterized), 44 (uncharacterized), 42 (dCMP-hydroxymethylase), and 56 (dCTPase) are also effective in small amounts. Some T4 gene products, such as the product of gene 41 (uncharacterized), seem to be partially dispensable for phage growth since am mutants of such genes do propagate, although weakly, in streptomycin-resistant su(-) hosts which appear to have lost the capacity to suppress am mutations by ambiguity.

MeSH Terms
Cell-Free System Chromatography, DEAE-Cellulose Coliphages/enzymology,growth & development,metabolism Crosses, Genetic Cytosine Nucleotides DNA Nucleotidyltransferases/biosynthesis,metabolism DNA Replication DNA Viruses/enzymology,growth & development,metabolism DNA, Viral/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects Mutation Phosphoric Monoester Hydrolases/metabolism Protein Biosynthesis Recombination, Genetic Streptomycin/pharmacology Suppression, Genetic Transferases/metabolism Virus Replication
Chemicals
Cytosine Nucleotides DNA, Viral Transferases DNA Nucleotidyltransferases Phosphoric Monoester Hydrolases Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karam J D
O'Donnell P V
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.6.933-945.1973
Published
1973-06-00
Pages
933-45
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355201
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.6.933-945.1973 PMC Full Text View journal details
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