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PMID: 4354857 Published · ppublish English Journal Article

Reexpression of the rat hypoxanthine phosphoribosyltransferase gene in rat-human hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 9 ·1973-09-00 ·Pages 2590-4

Croce CM, Bakay B, Nyhan WL, Koprowski H

Abstract

Fusion of hypoxanthine phosphoribosyltransferase (HPRT)(-) rat hepatoma cells with HPRT(+) human fibroblasts yielded hybrid clones that grew in HAT selective medium and contained all the rat chromosomes and one to nine human chromosomes. Among the retained chromosomes was the human X chromosome. In all clones backselected in medium containing 8-azaguanine, human X chromosome was absent. Electrophoretic analysis revealed that, without exception, hybrid clones growing in HAT medium had an active HPRT enzyme, either human or rat, or both. When these clones were backselected in 8-azaguanine, they did not show HPRT enzyme activity. Hybrids that contained the human X chromosome also had human glucose-6-phosphate dehydrogenase. The observed reexpression of rat HPRT in hybrid cells derived from HPRT(-) rat cells suggests that a genetic factor from the human cell determined the expression of the rat structural gene for HPRT.

MeSH Terms
Adenine Animals Carcinoma, Hepatocellular Cell Fusion Clone Cells Electrophoresis, Polyacrylamide Gel Female Fibroblasts Genes Glucosephosphate Dehydrogenase/analysis,biosynthesis Humans Hybrid Cells/enzymology Hypoxanthines Isoenzymes/analysis,biosynthesis Karyotyping Liver Neoplasms Parainfluenza Virus 1, Human Pentosyltransferases/analysis,biosynthesis Rats
Chemicals
Hypoxanthines Isoenzymes Glucosephosphate Dehydrogenase Pentosyltransferases Adenine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Croce C M
Bakay B
Nyhan W L
Koprowski H
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.9.2590
Published
1973-09-00
Pages
2590-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427062
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.9.2590 PMC Full Text View journal details
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