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PMID: 4358168 Published · ppublish English Journal Article

Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle.

Journal of virology ·Vol. 12 ·No. 5 ·1973-11-00 ·Pages 969-83

Repik P, Bishop DH

Abstract

A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T(1) to digest viral RNA labeled by (3)H-uridine, (3)H-cytidine, or (3)H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 +/- 0.14 x 10(6) whereas that of the VSV-defective T particle is estimated as 1.23 +/- 0.04 x 10(6).

MeSH Terms
Animals Cell Line Chromatography, DEAE-Cellulose Cricetinae Cytidine/metabolism Defective Viruses/analysis Guanosine/metabolism Hydrolysis Isotope Labeling Kidney Methods Molecular Weight Oligonucleotides/analysis Pancreas/enzymology Phosphorus Radioisotopes RNA, Viral/analysis,isolation & purification Ribonucleases Time Factors Tritium Uridine/metabolism Vesicular stomatitis Indiana virus/analysis,growth & development
Chemicals
Oligonucleotides Phosphorus Radioisotopes RNA, Viral Tritium Guanosine Cytidine Ribonucleases Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Repik P
Bishop D H
References (20)
20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.5.969-983.1973
Published
1973-11-00
Pages
969-83
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356726
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.5.969-983.1973 PMC Full Text View journal details
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