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PMID: 4358862 Published · ppublish English Journal Article

Inactivation of encephalomyocarditis virus in aerosols: fate of virus protein and ribonucleic acid.

Applied microbiology ·Vol. 27 ·No. 1 ·1974-01-00 ·Pages 59-65

de Jong JC, Harmsen M, Trouwborst T, Winkler KC

Abstract

After aerosolization at relative humidities of 50% or lower, encephalomyocarditis virus is rapidly inactivated. In this process the protein coat of the virion is damaged. This appears as a loss of hemagglutination activity and loss of affinity for hemagglutination inhibiting antibodies. The ribonucleic acid of the virus retains its infectivity but it becomes susceptible to ribonuclease. It sediments in sucrose gradients when centrifuged at high speed with the same velocity as free infectious ribonucleic acid extracted with phenol from intact encephalomyocarditis virus.

MeSH Terms
Aerosols Air Microbiology Animals Centrifugation, Density Gradient Dextrans/pharmacology Encephalomyocarditis virus/pathogenicity Hemagglutination Tests Hemagglutination, Viral Humidity L Cells Mice Phenols RNA, Viral/pharmacology Ribonucleases/pharmacology Sucrose Temperature Viral Proteins/pharmacology Virus Cultivation
Chemicals
Aerosols Dextrans Phenols RNA, Viral Viral Proteins Sucrose Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Jong J C
Harmsen M
Trouwborst T
Winkler K C
References (20)
20 references, click to expand
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Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.27.1.59-65.1974
Published
1974-01-00
Pages
59-65
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC379968
Subset
IM
External Links
PubMed source DOI: 10.1128/am.27.1.59-65.1974 PMC Full Text
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