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PMID: 4363249 Published · ppublish English Journal Article

Analysis of a viral agent isolated from multiple sclerosis brain tissue: characterization as a parainfluenzavirus type 1.

Journal of virology ·Vol. 13 ·No. 5 ·1974-05-00 ·Pages 1037-45

Lewandowski LJ, Lief FS, Verini MA, Pienkowski MM, ter Meulen V, Koprowski H

Abstract

A virus originally isolated from cell cultures obtained by lysolecithin-induced fusion of human multiple sclerosis brain cells with CV-1 cells has been analyzed for its antigenic, RNA, and polypeptide compositions, and for selective biological properties. Our findings establish that this isolate, designated 6/94 virus, contains a 50S RNA genome and is, as yet, indistinguishable from Sendai virus in its antigenic and total polypeptide compositions. Despite these similarities, the 6/94 and Sendai viruses differ in certain phenotypic properties. 6/94 virus is markedly less cytocidal for chick fibroblasts, especially at 37 C and, after beta-propiolactone inactivation, it possesses a greater capacity for cell fusion and a lower toxicity than does comparably treated Sendai virus. In addition, 6/94 virus shows greater hemolytic activity.

MeSH Terms
Animals Antigens, Viral/analysis Brain/microbiology,pathology Cell Fusion Cell Line Centrifugation, Density Gradient Chick Embryo Electrophoresis, Polyacrylamide Gel Haplorhini Hemolysis Humans Hybrid Cells/microbiology Kidney Multiple Sclerosis/microbiology Parainfluenza Virus 1, Human/analysis Peptides/analysis Respirovirus/analysis,isolation & purification Virus Cultivation
Chemicals
Antigens, Viral Peptides
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lewandowski L J
Lief F S
Verini M A
Pienkowski M M
ter Meulen V
Koprowski H
References (31)
31 references, click to expand
  1. Histopathology and electron microscopy of three cases of subacute sclerosing panencephalitis (SSPE).
    Acta Neuropathol. 1971;18(1):58-73 PMID: 4325445
  2. Virus-like structure in multiple sclerosis.
    Lancet. 1973 Sep 8;2(7828):569-70 PMID: 4125336
  3. Activation of a latent measles virus infection in hamster cells.
    J Virol. 1973 Oct;12(4):690-5 PMID: 4204692
  4. Virus-cell relationship in a carrier culture of HeLa cells and Coxsackie A9 virus.
    Virology. 1959 Jan;7(1):28-44 PMID: 13636054
  5. Selection of temperature-sensitive mutants during persistent infection: role in maintenance of persistent Newcastle disease virus infections of L cells.
    J Virol. 1973 Sep;12(3):481-91 PMID: 4795831
  6. Peristence of sindbis virus in BHK-21 cell cultures.
    Arch Gesamte Virusforsch. 1972;38(1):1-10 PMID: 5066350
  7. Interaction of Sendai (HVJ) virus with human erythrocytes: a morphological study of haemolysis cell fusion.
    J Gen Virol. 1972 Jun;15(3):227-34 PMID: 4114319
  8. Cells persistently infected with newcastle disease virus: I. Properties of mutants isolated from persistently infected L cells.
    J Virol. 1969 Sep;4(3):244-51 PMID: 16789100
  9. Properties of incomplete Sendai virions and subgenomic viral RNAs.
    Virology. 1970 Dec;42(4):857-71 PMID: 4099084
  10. Myxovirus ribonucleic acids.
    Annu Rev Microbiol. 1970;24:539-74 PMID: 4930373
  11. Temperature-sensitive mutants isolated from L cells persistently infected with Newcastle disease virus.
    J Virol. 1972 Feb;9(2):200-6 PMID: 5062677
  12. Localization of avian tumor virus group-specific antigen in cell and virus.
    Virology. 1966 Jul;29(3):377-84 PMID: 4289825
  13. Comparison of the structure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (diplornaviruses): cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus.
    J Virol. 1972 Nov;10(5):1053-70 PMID: 4636394
  14. Interactions between Sendai virus and human erythrocytes.
    J Virol. 1969 Jan;3(1):70-81 PMID: 4306303
  15. Temperature-sensitive phenomenon of viral maturation observed in BHK cells persistently infected with HVJ.
    Virology. 1972 Aug;49(2):453-61 PMID: 4559687
  16. Persistent infection of continuous line of pig kidney cells with a variant of the WSN strain of influenza A 0 virus.
    Proc Soc Exp Biol Med. 1972 May;140(1):109-17 PMID: 5033080
  17. Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus.
    J Virol. 1973 Sep;12(3):472-80 PMID: 4795830
  18. Acute multiple sclerosis: electron-microscopic evidence for and against a viral agent in the plaques.
    Lancet. 1973 Dec 22;2(7843):1408-11 PMID: 4128723
  19. Comparison of 6-94 virus and Sendai virus RNA by RNA-RNA hybridization.
    J Virol. 1974 Apr;13(4):935-6 PMID: 4362518
  20. Effect of environmental pH on the efficiency of cellular hybridization.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953-6 PMID: 4340167
  21. The nucleic acid of Sendai virus and ribonucleic acid synthesis in cells infected by Sendai virus.
    J Gen Virol. 1968 Jan;2(1):71-9 PMID: 4296360
  22. Electrophoretic distribution of the proteins and glycoproteins of influenza virus and Sendai virus.
    J Virol. 1970 Dec;6(6):707-16 PMID: 4322079
  23. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus.
    Virology. 1968 Aug;35(4):537-49 PMID: 4300362
  24. On the genesis of incomplete Sendai virions.
    Virology. 1970 Dec;42(4):872-9 PMID: 4321309
  25. Characterization of the subunit structure of the ribonucleic acid genome of influenza virus.
    J Virol. 1971 Nov;8(5):701-7 PMID: 4332140
  26. Paramyxovirus like tubules in multiple sclerosis biopsy material.
    Acta Neuropathol. 1973;25(4):281-90 PMID: 4584305
  27. Morphogenesis and structure of a virus in cells cultured from brain tissue from two cases of multiple sclerosis.
    Lab Invest. 1973 Apr;28(4):494-500 PMID: 4634094
  28. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  29. Acute multiple sclerosis. Confirmation of "paramyxovirus-like" intranuclear inclusions.
    Arch Neurol. 1974 Jan;30(1):39-46 PMID: 4808493
  30. Isolation of two plaque mutants of Western equine encephalitis virus differing in virulence for mice.
    J Virol. 1969 Nov;4(5):799-800 PMID: 5360528
  31. Paramyxovirus-like particles associated with acute demyelination in chronic relapsing multiple sclerosis.
    Science. 1972 Nov 17;178(4062):760-3 PMID: 5082841
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.5.1037-1045.1974
Published
1974-05-00
Pages
1037-45
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355412
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.5.1037-1045.1974 PMC Full Text View journal details
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