Effect of chemically modified 70S RNA from avian myeloblastosis virus (AMV) upon the activity of AMV DNA polymerase.

Journal of virology ·Vol. 14 ·No. 5 ·1974-11-00 ·Pages 1108-14

Papas TS, Chirikjian JG, Pry TW, Massicot JG, Irwin RD, Chirigos MA

Abstract

Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intact and was inactive as a template for the AMV DNA polymerase. Furthermore, it inhibited the reaction catalyzed by AMV polymerase when 70S RNA was used as template-primer and had no effect on the two tested bacterial polymerases. Protection against the 70S (epsilonA) RNA inhibition was observed when 70S RNA was primed with oligo(dT) indicating preference of the polymerase for the oligo(dT) primed regions.

MeSH Terms

Acetaldehyde/analogs & derivatives,pharmacology Avian Leukosis Virus/enzymology Carbon Radioisotopes Centrifugation, Density Gradient Chemical Phenomena Chemistry DNA Nucleotidyltransferases/antagonists & inhibitors Oligonucleotides/pharmacology RNA, Viral/analogs & derivatives,metabolism,pharmacology Spectrometry, Fluorescence Templates, Genetic Thymidine Transcription, Genetic Uridine

Chemicals

Carbon Radioisotopes Oligonucleotides RNA, Viral DNA Nucleotidyltransferases Acetaldehyde Thymidine Uridine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Papas T S

Chirikjian J G

Pry T W

Massicot J G

Irwin R D

Chirigos M A

References (11)

11 references, click to expand

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The 3'-terminal nucleosides of the high molecular weight RNA of avian myeloblastosis virus.
Proc Natl Acad Sci U S A. 1972 May;69(5):1176-80 PMID: 4338584
Synthesis and properties of a new fluorescent polynucleotide, poly(1,N6-ethenoadenylic acid).
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.14.5.1108-1114.1974

Published

1974-11-00

Pages

1108-14

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355627

Subset

External Links

PubMed source DOI: 10.1128/JVI.14.5.1108-1114.1974 PMC Full Text View journal details

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Effect of chemically modified 70S RNA from avian myeloblastosis virus (AMV) upon the activity of AMV DNA polymerase.

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