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PMID: 4373725 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Latent DNA of Epstein-Barr virus: separation from high-molecular-weight cell DNA in a neutral glycerol gradient.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4658-61

Tanaka A, Nonoyama M

Abstract

Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm(3) in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm(3). The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.

MeSH Terms
Burkitt Lymphoma Cell Line Centrifugation, Density Gradient DNA, Neoplasm/analysis DNA, Viral/isolation & purification Glycerol Herpesvirus 4, Human/analysis Humans Molecular Weight Nucleic Acid Hybridization RNA, Viral
Chemicals
DNA, Neoplasm DNA, Viral RNA, Viral Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tanaka A
Nonoyama M
References (9)
9 references, click to expand
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    Virology. 1970 Mar;40(3):776-9 PMID: 4314514
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  4. Occurrence of Epstein-Barr virus genomes in human lymphoblastoid cell lines.
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  5. MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. II. JOINING OF PARENTAL DNA MOLECULES OF PHAGE T4.
    J Mol Biol. 1964 Apr;8:516-40 PMID: 14153524
  6. Linear association between cellular DNA and Epstein-Barr virus DNA in a human lymphoblastoid cell line.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2888-92 PMID: 4355371
  7. Reassociation kinetics for Epstein-Barr virus DNA: nonhomology to mammalian DNA and homology of viral DNA in various diseases.
    J Virol. 1973 Nov;12(5):1006-12 PMID: 4358157
  8. Replication of the resident repressed Epstein-Barr virus genome during the early S phase (S-1 period) of nonproducer Raji cells.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):631-3 PMID: 4362625
  9. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.
    Nature. 1973 Mar 2;242(5392):44-7 PMID: 4348458
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4658
Published
1974-12-00
Pages
4658-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433954
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4658 PMC Full Text View journal details
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