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PMID: 4374934 Published · ppublish English Journal Article

The purification and amino acid sequence of cytochrome C-552 from Euglena gracilis.

The Biochemical journal ·Vol. 139 ·No. 2 ·1974-05-00 ·Pages 449-59

Pettigrew GW

Abstract

Cytochrome c-552 from Euglena gracilis was purified and the amino acid sequence determined. The protein is a single peptide chain of 87 residues with the haem prosthetic group bound through two thioether linkages to two cysteine residues near the amino-terminal region. The amino acid sequence shows some similarities to mitochondrial cytochrome c and to two prokaryote c-type cytochromes. The sequence, taken with the known characteristics of cytochrome c-552, indicates that it is an f-type cytochrome. The possible functional and evolutionary significance of these features in common is discussed. Detailed evidence for the amino acid sequence of Euglena cytochrome f has been deposited as Supplementary Publication SUP 50027 at the British Library, Lending Division (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7QB, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1973) 131, 5.

MeSH Terms
Amino Acid Sequence Centrifugation Chromatography, DEAE-Cellulose Chromatography, Gel Cysteine/analysis Cytochrome c Group/analysis,isolation & purification Cytochromes/analysis Electrophoresis, Polyacrylamide Gel Euglena gracilis/analysis Heme Hydroxylamines Mitochondria/analysis Tryptophan/analysis
Chemicals
Cytochrome c Group Cytochromes Hydroxylamines Heme Tryptophan Cysteine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pettigrew G W
References (31)
31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1390449
Published
1974-05-00
Pages
449-59
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166302
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1390449 PMC Full Text View journal details
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