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PMID: 4376941 Published · ppublish English Journal Article

Studies on chemical modification of thionucleosides in the transfer ribonucleic acid of Escherichia coli.

The Biochemical journal ·Vol. 143 ·No. 2 ·1974-11-00 ·Pages 285-94

Rao YS, Cherayil JD

Abstract

(35)S-labelled tRNA from Escherichia coli was treated with chemical reagents such as CNBr, H(2)O(2), NH(2)OH, I(2), HNO(2), KMnO(4) and NaIO(4), under mild conditions where the four major bases were not affected. Gel filtration of the treated tRNA showed desulphurization to various extents, depending on the nature of the reagent. The treated samples after conversion into nucleosides were chromatographed on a phosphocellulose column. NH(2)OH, I(2) and NaIO(4) reacted with all the four thionucleosides of E. coli tRNA, 4-thiouridine (s(4)U), 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U), 2-thiocytidine (s(2)C) and 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A), to various extents. CNBr, HNO(2) and NaHSO(3) reacted with s(4)U, mnm(5)s(2)U and s(2)C, but not with ms(2)i(6)A. KMnO(4) and H(2)O(2) were also found to react extensively with thionucleosides in tRNA. Iodine oxidation of (35)S-labelled tRNA showed that only 6% of the sulphur was involved in disulphide formation. Desulphurization of E. coli tRNA with CNBr resulted in marked loss of acceptor activities for glutamic acid, glutamine and lysine. Acceptor activities for alanine, arginine, glycine, isoleucine, methionine, phenylalanine, serine, tyrosine and valine were also affected, but to a lesser extent. Five other amino acids tested were almost unaffected. These results indicate the fate of thionucleosides in tRNA when subjected to various chemical reactions and the involvement of sulphur in aminoacyl-tRNA synthetase recognition of some tRNA species of E. coli.

MeSH Terms
Chromatography, Gel Cyanogen Bromide Cytidine Escherichia coli/analysis Hydrogen Peroxide Hydroxylamines Nitrous Acid Nucleosides Oxidation-Reduction Periodic Acid Potassium Permanganate RNA, Transfer/isolation & purification S-Adenosylmethionine Sulfides/analysis Sulfites Sulfur Radioisotopes Thiouridine
Chemicals
Hydroxylamines Nucleosides Sulfides Sulfites Sulfur Radioisotopes Potassium Permanganate Periodic Acid Thiouridine Cytidine S-Adenosylmethionine RNA, Transfer Hydrogen Peroxide Cyanogen Bromide Nitrous Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rao Y S
Cherayil J D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1430285
Published
1974-11-00
Pages
285-94
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168383
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1430285 PMC Full Text View journal details
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