Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the "dopamine receptor".

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2145-9

Kebabian JW, Petzold GL, Greengard P

Abstract

An adenylate cyclase that is activated specifically by low concentrations of dopamine has been demonstrated in homogenates of caudate nucleus of rat brain. A half-maximal increase in the activity of the enzyme occurred in the presence of 4 muM dopamine. Concentrations of dopamine as low as 0.3 muM stimulated the activity of the enzyme. The adenylate cyclase activity of the homogenates was also stimulated by low concentrations of apomorphine, a substance known to mimic the physiological and pharmacological effects of dopamine. The stimulatory effect of dopamine was blocked by low concentrations of either haloperidol or chlorpromazine, agents known to block the actions of dopamine in mammalian brain. The results suggest that dopamine-sensitive adenylate cyclase may be the receptor for dopamine in mammalian brain. The isolation of this enzyme from caudate nucleus should facilitate the search for new therapeutic agents useful in the treatment of extrapyramidal diseases.

MeSH Terms

Adenylyl Cyclases/isolation & purification Adrenergic alpha-Antagonists/pharmacology Animals Apomorphine/pharmacology Caudate Nucleus/drug effects,enzymology Chlorpromazine/pharmacology Cyclic AMP/analysis Dopamine/pharmacology Dopamine Antagonists Enzyme Activation/drug effects Haloperidol/pharmacology Isoproterenol/pharmacology Male Methods Norepinephrine/pharmacology Phentolamine/pharmacology Rats Rats, Inbred Strains Receptors, Drug

Chemicals

Adrenergic alpha-Antagonists Dopamine Antagonists Receptors, Drug Cyclic AMP Adenylyl Cyclases Haloperidol Isoproterenol Apomorphine Chlorpromazine Dopamine Norepinephrine Phentolamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kebabian J W

Petzold G L

Greengard P

References (20)

20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.8.2145

Published

1972-08-00

Pages

2145-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426888

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.8.2145 PMC Full Text View journal details

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Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the "dopamine receptor".

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