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PMID: 4401122 Published · ppublish English Journal Article

Stimulation by dopamine of adenylate cyclase in retinal homogenates and of adenosine-3':5'-cyclic monophosphate formation in intact retina.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 3 ·1972-03-00 ·Pages 539-43

Brown JH, Makman MH

Abstract

A catecholamine-sensitive adenylate cyclase system is present in homogenates of both calf and rat retinas. Dopamine is a more potent activator of the bovine enzyme than is norepinephrine or epinephrine. Cyclic AMP concentrations in intact bovine retina are increased by dopamine, as well as by other catecholamines, and by depolarizing agents. Studies with adrenergic blocking agents suggest that the stimulation of retinal adenylate cyclase by catecholamines cannot be clearly defined in terms of the characteristics of alpha or beta adrenergic receptors. Bovine retina also contains a protein kinase that is stimulated 20-fold by cyclic AMP. It is proposed that dopamine is the major activator of a retinal adenylate cyclase, and that this activation is related to its role as a neurotransmitter.

MeSH Terms
Adenylyl Cyclases/analysis,metabolism Animals Cattle Cyclic AMP/analysis,biosynthesis Dopamine/pharmacology Enzyme Activation Epinephrine/pharmacology In Vitro Techniques Isoproterenol/pharmacology Male Norepinephrine/pharmacology Ouabain/pharmacology Phenoxybenzamine/pharmacology Phentolamine/pharmacology Phosphotransferases/analysis Propranolol/pharmacology Proteins Rats Retina/analysis,enzymology,metabolism
Chemicals
Proteins Phenoxybenzamine Ouabain Propranolol Cyclic AMP Phosphotransferases Adenylyl Cyclases Isoproterenol Dopamine Norepinephrine Epinephrine Phentolamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown J H
Makman M H
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.3.539
Published
1972-03-00
Pages
539-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426502
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.3.539 PMC Full Text View journal details
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