Induction and superinduction of serotonin N-acetyltransferase by adrenergic drugs and denervation in rat pineal organ.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2208-11

Deguchi T, Axelrod J

Abstract

Activity of serotonin N-acetyltransferase (EC 2.3.1.5) in rat pineal organ is rapidly and markedly elevated in vivo after administration of beta-(3,4-dihydroxyphenyl)-L-alanine (L-DOPA), norepinephrine, epinephrine, isoproterenol, monoamine oxidase inhibitors, or theophylline. Serotonin or 5-hydroxytryptophan has no effect on the increase in activity of this enzyme. Inhibitors of protein synthesis or propranolol, a beta-adrenergic blocking agent completely inhibit(s) the increase in activity of serotonin N-acetyltransferase induced by drugs, indicating that new enzyme molecules are formed via stimulation of beta-receptors of pineal cells and adenosine 3':5'-cyclic monophosphate. When rat pineal organ is denervated by ganglionectomy, beta-(3,4-dihydroxyphenyl)-L-alanine induces much more serotonin N-acetyltransferase than in the innervated gland. This superinduction by denervation appears to be due to changes of the postsynaptic site, probably the beta-adrenergic receptor on the pineal cell.

MeSH Terms

5-Hydroxytryptophan/pharmacology Acyltransferases/analysis,antagonists & inhibitors Adrenergic beta-Antagonists/pharmacology Animals Cycloheximide/pharmacology Dactinomycin/pharmacology Denervation Dihydroxyphenylalanine/pharmacology Enzyme Induction/drug effects Epinephrine/pharmacology Isoproterenol/pharmacology Male Monoamine Oxidase Inhibitors/pharmacology Norepinephrine/pharmacology Phenoxybenzamine/pharmacology Pineal Gland/drug effects,enzymology Propranolol/pharmacology Rats Receptors, Adrenergic/drug effects Serotonin/pharmacology Theophylline/pharmacology

Chemicals

Adrenergic beta-Antagonists Monoamine Oxidase Inhibitors Receptors, Adrenergic Phenoxybenzamine Dactinomycin Serotonin Dihydroxyphenylalanine Cycloheximide Propranolol Theophylline 5-Hydroxytryptophan Acyltransferases Isoproterenol Norepinephrine Epinephrine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Deguchi T

Axelrod J

References (20)

20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.8.2208

Published

1972-08-00

Pages

2208-11

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426901

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.8.2208 PMC Full Text View journal details

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Induction and superinduction of serotonin N-acetyltransferase by adrenergic drugs and denervation in rat pineal organ.

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