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PMID: 440966 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The DNA polymerases associated with the adenovirus type 2 replication complex: effect of 2'-3'-dideoxythymidine-5'-triphosphate on viral DNA synthesis.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 1025-39

Abboud MM, Horwitz MS

Abstract

An adenovirus (Ad) DNA replication complex extracted from infected HeLa nuclei could be purified free of the bulk of intracellular DNA polymerase activity by sedimetation in neutral sucrose gradients. However, the replication complex still retained some alpha and gamma DNA-polymerase activity. Since this complex is inhibited by 2', 3' dideoxythymidine-5'-triphosphate (ddTTP), an inhibitor of DNA polymerase gamma, a functional role for this enzyme in Ad DNA replication is suggested. Similar inhibition by ddTTP in intact Ad infected nuclei and comparable inhibition of Ad DNA synthesis in whole cells by dideoxythymidine (ddThy) are consistent with a role for DNA polymerase gamma. Uninfected HeLa nuclei or whole cells are not similarly inhibited by ddTTP or DDThy respectively. Such data does not rule out an additional functional role for other DNA polymerases, and recent experiments from this laboratory (1) suggest that DNA polymerase alpha is also involved in Ad DNA synthesis.

MeSH Terms
Adenoviruses, Human/enzymology Cell Nucleus/enzymology DNA Replication DNA, Viral/biosynthesis DNA-Directed DNA Polymerase/isolation & purification,metabolism HeLa Cells Humans Kinetics Thymidine/analogs & derivatives,pharmacology Thymine Nucleotides/pharmacology Virus Replication
Chemicals
DNA, Viral Thymine Nucleotides DNA-Directed DNA Polymerase Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Abboud M M
Horwitz M S
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.3.1025
Published
1979-03-00
Pages
1025-39
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327750
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.3.1025 PMC Full Text View journal details
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