Evidence for a cytolytic factor released by macrophages.
Mouse peritoneal macrophages cultivated in vitro acquire a strong extracellular cytotoxic activity towards isotope labeled syngeneic erythrocytes as demonstrated by isotope release to the medium. This lytic process is mediated by an extremely labile macrophage cytolytic factor (MCF) which is not detected under ordinary tissue culture conditions with serum present in the medium. By the use of serum-free medium containing low doses of 2-mercaptoethanol MCF is stabilized and found to be an easily dialysable, low molecular substance which resists heating at 60 degrees C for 30 min.
14 references, click to expand
-
Fine structures of capillary and endocapillary layer as revealed by ruthenium red.Fed Proc. 1966 Nov-Dec;25(6):1773-83 PMID: 5927412
-
Red cells coated with immunoglobulin G: binding and sphering by mononuclear cells in man.Science. 1967 Dec 22;158(3808):1582-5 PMID: 4168593
-
Enhancement by reducing agents of the transformation of human and rabbit peripheral lymphocytes.J Immunol. 1970 Oct;105(4):1043-5 PMID: 5470518
-
Ultrastructural visualization of cellular carbohydrate components by means of concanavalin A.Exp Cell Res. 1971 Jan;64(1):232-6 PMID: 5541973
-
Cytotoxic factor from peritoneal cells: purification and characteristics.J Reticuloendothel Soc. 1970 Dec;8(6):508-21 PMID: 5500454
-
Cytotoxic interaction of phytohemagglutinin-stimulated blood lymphocytes with monolayer cells: a study by light and electron microscopy.Cell Immunol. 1971 Feb;2(1):54-72 PMID: 4940897
-
Lymphocyte-activating factors released in vitro by sensitized and nonsensitized human lymphocytes.Cell Immunol. 1970 Jul;1(2):150-61 PMID: 4943426
-
The in vitro induction and release of a cell toxin by immune C57B1-6 mouse peritoneal macrophages.Cell Immunol. 1972 Jan;3(1):88-100 PMID: 5009049
-
The effects of mercaptoethanol and of peritoneal macrophages on the antibody-forming capacity of nonadherent mouse spleen cells in vitro.J Exp Med. 1972 Sep 1;136(3):604-17 PMID: 4559193
-
The cytotoxic effect of mouse macrophages on syngeneic and allogeneic erythrocytes.J Exp Med. 1973 Mar 1;137(3):807-20 PMID: 4570019
-
Detection of macrophage receptors for heterologous IgG by scanning and transmission electron microscopy.J Pathol. 1973 Jul;110(3):213-20 PMID: 4744066
-
The cytotoxic effect of macrophages studied by time-lapse microcinematography.Exp Cell Res. 1973 Aug;80(2):388-92 PMID: 4583192
-
Cytotoxic activity of mouse macrophages studied by various inhibitors.J Exp Med. 1974 May 1;139(5):1049-60 PMID: 4825241
-
Rifamycin--a potent inhibitor of macrophage cytotoxicity.Exp Cell Res. 1974 Jun;86(2):415-9 PMID: 4276370
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