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PMID: 448802 Published · ppublish English Journal Article

Purification and characterization of an early glycoprotein from adenovirus type 2-infected cells.

Journal of virology ·Vol. 29 ·No. 3 ·1979-03-00 ·Pages 938-48

Persson H, Signäs C, Philipson L

Abstract

An adenovirus type 2 early glycoprotein with an apparent molecular weight of 19,000 (E19K) in sodium dodecyl sulfate-polyacrylamide gels has been extensively purified. Purification involved detergent solubilization of membrane fractions from infected cells, followed by affinity chromatography on a lectin column and DEAE-Sephadex chromatography. The purified material contained three polypeptides (E40K, E19K, E17.5K), with approximately 90% of the material in the E19K moiety. All three polypeptides yielded identical tryptic peptide maps. The E19K polypeptide contained glucosamine as revealed by [3H]glucosamine labeling of infected cells and amino acid analysis of the purified protein. Immunoprecipitation with a monospecific antiserum showed that the E19K polypeptide started to be synthesized at 2 h, with a maximal rate at 4 h after infection. It was also synthesized at a low rate late in the infectious cycle (12 to 24 h postinfection). Immunoprecipitation from three adenovirus type 2-transformed hamster embryo cell lines and two adenovirus type 2-transformed rat cell lines revealed that one of the hamster cell lines (ad2HE4) and one of the rat cell lines (A2T2C4) expressed this protein.

MeSH Terms
Adenoviruses, Human/analysis,metabolism Amino Acids/analysis Animals Cricetinae Glycoproteins/analysis,isolation & purification HeLa Cells Molecular Weight Peptide Biosynthesis Peptides/analysis Rats Viral Proteins/analysis,biosynthesis,isolation & purification
Chemicals
Amino Acids Glycoproteins Peptides Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Persson H
Signäs C
Philipson L
References (30)
30 references, click to expand
  1. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  2. Purification and molecular characterization of adenovirus type 2 DNA-binding protein.
    J Virol. 1977 Jan;21(1):338-46 PMID: 64628
  3. Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4291-5 PMID: 212742
  4. Purification and characterization of the phosphorylated DNA-binding protein from adenovirus-type-2-infected cells.
    Eur J Biochem. 1977 Jun 15;76(2):481-90 PMID: 408135
  5. Molecular association between transplantation antigens and cell surface antigen in adenovirus-transformed cell line.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5674-8 PMID: 310123
  6. The concept that surveillance of self is mediated via the same set of genes that determines recognition of allogenic cells.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:505-10 PMID: 302174
  7. Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2.
    J Virol. 1978 Sep;27(3):628-39 PMID: 568181
  8. Synthesis of a structural adenovirus polypeptide in the absence of viral DNA replication.
    Virology. 1978 Oct 1;90(1):67-79 PMID: 716228
  9. Purification and characterization of an early protein (E14K) from adenovirus type 2-infected cells.
    J Virol. 1978 Oct;28(1):119-39 PMID: 702645
  10. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  11. Adenovirus type 2 early proteins synthesized in vitro and in vivo: identification in infected cells of the 38,000- to 50,000- molecular-weight protein encoded by the left end of the adenovirus type 2 genome.
    J Virol. 1978 Jun;26(3):736-49 PMID: 671586
  12. Ultraviolet mapping of the adenovirus 2 early promoters.
    Cell. 1977 Sep;12(1):45-55 PMID: 902320
  13. Early gene expression of adenovirus type 2: R-loop mapping of mRNA and time course of viral DNA, mRNA, and protein synthesis.
    J Virol. 1977 Mar;21(3):1019-30 PMID: 850303
  14. The topography and transcription of the adenovirus genome.
    Cell. 1977 Feb;10(2):153-66 PMID: 837446
  15. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. Transformation of hamster kidney cells by adenovirus 2 and the nondefective hybrid viruses.
    J Virol. 1974 Jun;13(6):1291-301 PMID: 4364901
  16. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;.
    J Mol Biol. 1974 Oct 15;89(1):49-72 PMID: 4613857
  17. Molecular biology of adenoviruses.
    Virol Monogr. 1975;14:1-115 PMID: 170728
  18. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  19. Effect of cycloheximide on RNA metabolism early in productive infection with adenovirus 2.
    J Virol. 1974 Jul;14(1):26-32 PMID: 4836598
  20. The polypeptides of adenovirus. VI. Early and late glycopolypeptides.
    Virology. 1974 Apr;58(2):345-61 PMID: 4856570
  21. The polypeptides of adenovirus. IV. Detection of early and late virus-induced polypeptides and their distribution in subcellular fractions.
    Virology. 1974 Feb;57(2):402-8 PMID: 4817073
  22. Processing of adenovirus 2-induced proteins.
    J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
  23. Lectins: cell-agglutinating and sugar-specific proteins.
    Science. 1972 Sep 15;177(4053):949-59 PMID: 5055944
  24. Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin.
    Biochem Biophys Res Commun. 1972 May 26;47(4):923-30 PMID: 5026302
  25. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  26. Location and identification of the genes for adenovirus type 2 early polypeptides.
    Cell. 1976 Jan;7(1):141-51 PMID: 949741
  27. Detection of adenovirus type 2-induced early polypeptides using cycloheximide pretreatment to enhance viral protein synthesis.
    J Virol. 1976 Jul;19(1):232-42 PMID: 950686
  28. In vivo and in vitro synthesis of adenovirus type 2 early proteins.
    J Virol. 1976 Mar;17(3):865-75 PMID: 1255861
  29. The polypeptides of adenovirus. VII. Further studies of early polypeptides in vivo and localization of E2 and E2A to the cell plasma membrane.
    Virology. 1976 Jun;71(2):518-30 PMID: 936473
  30. Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells.
    Virology. 1971 Jul;45(1):154-62 PMID: 5165249
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.3.938-948.1979
Published
1979-03-00
Pages
938-48
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353253
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.3.938-948.1979 PMC Full Text View journal details
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