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PMID: 310123 Published · ppublish English Journal Article

Molecular association between transplantation antigens and cell surface antigen in adenovirus-transformed cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 11 ·1978-11-00 ·Pages 5674-8

Kvist S, Ostberg L, Persson H, Philipson L, Peterson PA

Abstract

A rat cell line (A2T2C4) transformed with adenovirus type 2 elicited cytotoxic T lymphocytes in syngeneic rats. Cytotoxicity was abolished by a rabbit antiserum directed against the major histocompatibility (AgB) antigens and by a syngeneic rat antiserum raised against the virus-transformed cell line. The syngeneic antiserum immunoprecipitated surface proteins with apparent molecular weights of 45,000, 19,000, 17,000, and 12,000 from the A2T2C4 cells but it displayed no reactivity against primary rat fibroblasts and spleen cells. The rabbit antiserum against AgB antigens precipitated a 19,000-dalton component from the A2T2C4 cells which was not observed in primary rat fibroblasts. Sequential immunoprecipitation revealed identity between the major polypeptides recognized by the two antisera. Because the rabbit anti-AgB antigen serum was specific for the transplantation antigen subunits and because the syngeneic rat antiserum against the A2T2C4 cells failed to react with the AgB antigens in normal cells, it is concluded that the 19,000-dalton component is coprecipitated with the AgB antigens. Antisera directed specifically against beta2-microglobulin and the alloantigenic AgB antigen subunit also coprecipitated the 19,000-dalton component. These results indicate that the AgB antigen subunits form a ternary complex with a virus-coded protein on the surface of the virus-transformed A2T2C4 cells. This molecular complex may be recognized by the cytoloytic T lymphocytes

MeSH Terms
Adenoviruses, Human Animals Antigens, Surface Cell Line Cell Transformation, Viral HLA Antigens Macromolecular Substances Male Molecular Weight Precipitin Tests Rats T-Lymphocytes/immunology Transplantation Immunology
Chemicals
Antigens, Surface HLA Antigens Macromolecular Substances
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kvist S
Ostberg L
Persson H
Philipson L
Peterson P A
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.11.5674
Published
1978-11-00
Pages
5674-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393030
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.11.5674 PMC Full Text View journal details
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