Sialic acids on the plasma membrane of cultured human lymphoid cells. Chemical aspects and biosynthesis.

The Journal of cell biology ·Vol. 53 ·No. 2 ·1972-05-00 ·Pages 466-73

Rosenberg SA, Einstein AB

Abstract

From 61 to 92% of the total sialic acid of a variety of human lymphoid cell lines maintained in tissue culture is present on the cell surface as measured by its susceptibility to cleavage by Clostridium perfringens neuraminidase. These cells contain from 1.22 x 10(8) to 6.99 x 10(8) molecules of surface sialic acid per cell. In synchronized cultures synthesis of surface sialic acid occurs only during a limited time in the late G(2) phase of the cell cycle. The amount and density of surface sialic acid vary considerably throughout the cell cycle.

MeSH Terms

Burkitt Lymphoma Cell Count Cell Division Cell Line Cell Membrane/analysis,metabolism Cells, Cultured DNA/analysis Humans Hydrogen-Ion Concentration Kinetics Leukemia, Myeloid Leukemia, Myeloid, Acute Lymphoid Tissue/analysis,cytology Lymphoma Mitosis/drug effects Multiple Myeloma Neuraminic Acids/analysis,biosynthesis Neuraminidase Plasmacytoma Thymidine/pharmacology Time Factors

Chemicals

Neuraminic Acids DNA Neuraminidase Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rosenberg S A

Einstein A B

References (18)

18 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.53.2.466

Published

1972-05-00

Pages

466-73

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108726

Subset

External Links

PubMed source DOI: 10.1083/jcb.53.2.466 PMC Full Text View journal details

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Sialic acids on the plasma membrane of cultured human lymphoid cells. Chemical aspects and biosynthesis.

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